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Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1
Size and copy number of organelles are influenced by an equilibrium of membrane fusion and fission. We studied this equilibrium on vacuoles—the lysosomes of yeast. Vacuole fusion can readily be reconstituted and quantified in vitro, but it had not been possible to study fission of the organelle in a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290646/ https://www.ncbi.nlm.nih.gov/pubmed/22238359 http://dx.doi.org/10.1091/mbc.E11-08-0703 |