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Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1

Size and copy number of organelles are influenced by an equilibrium of membrane fusion and fission. We studied this equilibrium on vacuoles—the lysosomes of yeast. Vacuole fusion can readily be reconstituted and quantified in vitro, but it had not been possible to study fission of the organelle in a...

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Detalles Bibliográficos
Autores principales: Michaillat, Lydie, Baars, Tonie Luise, Mayer, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290646/
https://www.ncbi.nlm.nih.gov/pubmed/22238359
http://dx.doi.org/10.1091/mbc.E11-08-0703