Detection of Infectious Poxvirus Particles

To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect <3 PFU of infectious poxvirus particles in <5 hours. This approach involved virus culture combined with real-time reverse transcription–polymerase chain r...

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Detalles Bibliográficos
Autores principales: Nitsche, Andreas, Stern, Daniel, Ellerbrok, Heinz, Pauli, Georg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2006
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291062/
https://www.ncbi.nlm.nih.gov/pubmed/16836836
http://dx.doi.org/10.3201/eid1207.060093
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author Nitsche, Andreas
Stern, Daniel
Ellerbrok, Heinz
Pauli, Georg
author_facet Nitsche, Andreas
Stern, Daniel
Ellerbrok, Heinz
Pauli, Georg
author_sort Nitsche, Andreas
collection PubMed
description To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect <3 PFU of infectious poxvirus particles in <5 hours. This approach involved virus culture combined with real-time reverse transcription–polymerase chain reaction detection of 2 viral genes expressed immediately after infection.
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spelling pubmed-32910622012-03-05 Detection of Infectious Poxvirus Particles Nitsche, Andreas Stern, Daniel Ellerbrok, Heinz Pauli, Georg Emerg Infect Dis Dispatch To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect <3 PFU of infectious poxvirus particles in <5 hours. This approach involved virus culture combined with real-time reverse transcription–polymerase chain reaction detection of 2 viral genes expressed immediately after infection. Centers for Disease Control and Prevention 2006-07 /pmc/articles/PMC3291062/ /pubmed/16836836 http://dx.doi.org/10.3201/eid1207.060093 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Nitsche, Andreas
Stern, Daniel
Ellerbrok, Heinz
Pauli, Georg
Detection of Infectious Poxvirus Particles
title Detection of Infectious Poxvirus Particles
title_full Detection of Infectious Poxvirus Particles
title_fullStr Detection of Infectious Poxvirus Particles
title_full_unstemmed Detection of Infectious Poxvirus Particles
title_short Detection of Infectious Poxvirus Particles
title_sort detection of infectious poxvirus particles
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291062/
https://www.ncbi.nlm.nih.gov/pubmed/16836836
http://dx.doi.org/10.3201/eid1207.060093
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