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Phyllanthus urinaria Induces Apoptosis in Human Osteosarcoma 143B Cells via Activation of Fas/FasL- and Mitochondria-Mediated Pathways
Phyllanthus urinaria (P. urinaria), in this study, was used for the treatment of human osteosarcoma cells, which is one of the tough malignancies with few therapeutic modalities. Herein, we demonstrated that P. urinaria inhibited human osteosarcoma 143B cells growth through an apoptotic extrinsic pa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291129/ https://www.ncbi.nlm.nih.gov/pubmed/22454688 http://dx.doi.org/10.1155/2012/925824 |
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author | Wu, Hsin-Yi Lin, Tsu-Kung Kuo, Hsiao-Mei Huang, Ya-Ling Liou, Chia-Wei Wang, Pei-Wen Chuang, Jiin-Haur Huang, Sheng-Teng |
author_facet | Wu, Hsin-Yi Lin, Tsu-Kung Kuo, Hsiao-Mei Huang, Ya-Ling Liou, Chia-Wei Wang, Pei-Wen Chuang, Jiin-Haur Huang, Sheng-Teng |
author_sort | Wu, Hsin-Yi |
collection | PubMed |
description | Phyllanthus urinaria (P. urinaria), in this study, was used for the treatment of human osteosarcoma cells, which is one of the tough malignancies with few therapeutic modalities. Herein, we demonstrated that P. urinaria inhibited human osteosarcoma 143B cells growth through an apoptotic extrinsic pathway to activate Fas receptor/ligand expression. Both intracellular and mitochondrial reactive oxygen species were increased to lead to alterations of mitochondrial membrane permeability and Bcl-2 family including upregulation of Bid, tBid, and Bax and downregulation of Bcl-2. P. urinaria triggered an intrinsic pathway and amplified the caspase cascade to induce apoptosis of 143B cells. However, upregulation of both intracellular and mitochondrial reactive oxygen species and the sequential membrane potential change were less pronounced in the mitochondrial respiratory-defective 143Bρ (0) cells compared with the 143B cells. This study offers the evidence that mitochondria are essential for the anticancer mechanism induced by P. urinaria through both extrinsic and intrinsic pathways. |
format | Online Article Text |
id | pubmed-3291129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-32911292012-03-27 Phyllanthus urinaria Induces Apoptosis in Human Osteosarcoma 143B Cells via Activation of Fas/FasL- and Mitochondria-Mediated Pathways Wu, Hsin-Yi Lin, Tsu-Kung Kuo, Hsiao-Mei Huang, Ya-Ling Liou, Chia-Wei Wang, Pei-Wen Chuang, Jiin-Haur Huang, Sheng-Teng Evid Based Complement Alternat Med Research Article Phyllanthus urinaria (P. urinaria), in this study, was used for the treatment of human osteosarcoma cells, which is one of the tough malignancies with few therapeutic modalities. Herein, we demonstrated that P. urinaria inhibited human osteosarcoma 143B cells growth through an apoptotic extrinsic pathway to activate Fas receptor/ligand expression. Both intracellular and mitochondrial reactive oxygen species were increased to lead to alterations of mitochondrial membrane permeability and Bcl-2 family including upregulation of Bid, tBid, and Bax and downregulation of Bcl-2. P. urinaria triggered an intrinsic pathway and amplified the caspase cascade to induce apoptosis of 143B cells. However, upregulation of both intracellular and mitochondrial reactive oxygen species and the sequential membrane potential change were less pronounced in the mitochondrial respiratory-defective 143Bρ (0) cells compared with the 143B cells. This study offers the evidence that mitochondria are essential for the anticancer mechanism induced by P. urinaria through both extrinsic and intrinsic pathways. Hindawi Publishing Corporation 2012 2012-02-20 /pmc/articles/PMC3291129/ /pubmed/22454688 http://dx.doi.org/10.1155/2012/925824 Text en Copyright © 2012 Hsin-Yi Wu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wu, Hsin-Yi Lin, Tsu-Kung Kuo, Hsiao-Mei Huang, Ya-Ling Liou, Chia-Wei Wang, Pei-Wen Chuang, Jiin-Haur Huang, Sheng-Teng Phyllanthus urinaria Induces Apoptosis in Human Osteosarcoma 143B Cells via Activation of Fas/FasL- and Mitochondria-Mediated Pathways |
title |
Phyllanthus urinaria Induces Apoptosis in Human Osteosarcoma 143B Cells via Activation of Fas/FasL- and Mitochondria-Mediated Pathways |
title_full |
Phyllanthus urinaria Induces Apoptosis in Human Osteosarcoma 143B Cells via Activation of Fas/FasL- and Mitochondria-Mediated Pathways |
title_fullStr |
Phyllanthus urinaria Induces Apoptosis in Human Osteosarcoma 143B Cells via Activation of Fas/FasL- and Mitochondria-Mediated Pathways |
title_full_unstemmed |
Phyllanthus urinaria Induces Apoptosis in Human Osteosarcoma 143B Cells via Activation of Fas/FasL- and Mitochondria-Mediated Pathways |
title_short |
Phyllanthus urinaria Induces Apoptosis in Human Osteosarcoma 143B Cells via Activation of Fas/FasL- and Mitochondria-Mediated Pathways |
title_sort | phyllanthus urinaria induces apoptosis in human osteosarcoma 143b cells via activation of fas/fasl- and mitochondria-mediated pathways |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291129/ https://www.ncbi.nlm.nih.gov/pubmed/22454688 http://dx.doi.org/10.1155/2012/925824 |
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