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SARS–associated Coronavirus Replication in Cell Lines

Given the potential for laboratory-associated severe acute respiratory syndrome–associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. We investigated 21 cell lines routinely used for virus isolation or research. After infection with SARS-CoV, cells...

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Autores principales: Kaye, Matthew, Druce, Julian, Tran, Thomas, Kostecki, Renata, Chibo, Doris, Morris, Jessica, Catton, Mike, Birch, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291385/
https://www.ncbi.nlm.nih.gov/pubmed/16494729
http://dx.doi.org/10.3201/eid1201.050496
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author Kaye, Matthew
Druce, Julian
Tran, Thomas
Kostecki, Renata
Chibo, Doris
Morris, Jessica
Catton, Mike
Birch, Chris
author_facet Kaye, Matthew
Druce, Julian
Tran, Thomas
Kostecki, Renata
Chibo, Doris
Morris, Jessica
Catton, Mike
Birch, Chris
author_sort Kaye, Matthew
collection PubMed
description Given the potential for laboratory-associated severe acute respiratory syndrome–associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. We investigated 21 cell lines routinely used for virus isolation or research. After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. An indirect immunofluorescence assay was also used as a confirmatory test. The study identified 10 new cell lines capable of supporting the replication of SARS-CoV and confirmed the susceptibility of 4 cell lines previously reported. This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes. It also shows that SARS-CoV can achieve high titers in several cell lines, sometimes in the absence of specific cytopathic effects.
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spelling pubmed-32913852012-03-05 SARS–associated Coronavirus Replication in Cell Lines Kaye, Matthew Druce, Julian Tran, Thomas Kostecki, Renata Chibo, Doris Morris, Jessica Catton, Mike Birch, Chris Emerg Infect Dis Research Given the potential for laboratory-associated severe acute respiratory syndrome–associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. We investigated 21 cell lines routinely used for virus isolation or research. After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. An indirect immunofluorescence assay was also used as a confirmatory test. The study identified 10 new cell lines capable of supporting the replication of SARS-CoV and confirmed the susceptibility of 4 cell lines previously reported. This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes. It also shows that SARS-CoV can achieve high titers in several cell lines, sometimes in the absence of specific cytopathic effects. Centers for Disease Control and Prevention 2006-01 /pmc/articles/PMC3291385/ /pubmed/16494729 http://dx.doi.org/10.3201/eid1201.050496 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Kaye, Matthew
Druce, Julian
Tran, Thomas
Kostecki, Renata
Chibo, Doris
Morris, Jessica
Catton, Mike
Birch, Chris
SARS–associated Coronavirus Replication in Cell Lines
title SARS–associated Coronavirus Replication in Cell Lines
title_full SARS–associated Coronavirus Replication in Cell Lines
title_fullStr SARS–associated Coronavirus Replication in Cell Lines
title_full_unstemmed SARS–associated Coronavirus Replication in Cell Lines
title_short SARS–associated Coronavirus Replication in Cell Lines
title_sort sars–associated coronavirus replication in cell lines
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291385/
https://www.ncbi.nlm.nih.gov/pubmed/16494729
http://dx.doi.org/10.3201/eid1201.050496
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