Preparation of Mouse Monoclonal Antibody for RB1CC1 and Its Clinical Application

RB1-inducible coiled-coil 1 (RB1CC1; also known as FIP200) plays important roles in several biological pathways such as cell proliferation and autophagy. Evaluation of RB1CC1 expression can provide useful clinical information on various cancers and neurodegenerative diseases. In order to realize the...

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Autores principales: Hama, Yusuke, Chano, Tokuhiro, Inui, Takuma, Matsumoto, Kyoichi, Okabe, Hidetoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291565/
https://www.ncbi.nlm.nih.gov/pubmed/22396748
http://dx.doi.org/10.1371/journal.pone.0032052
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author Hama, Yusuke
Chano, Tokuhiro
Inui, Takuma
Matsumoto, Kyoichi
Okabe, Hidetoshi
author_facet Hama, Yusuke
Chano, Tokuhiro
Inui, Takuma
Matsumoto, Kyoichi
Okabe, Hidetoshi
author_sort Hama, Yusuke
collection PubMed
description RB1-inducible coiled-coil 1 (RB1CC1; also known as FIP200) plays important roles in several biological pathways such as cell proliferation and autophagy. Evaluation of RB1CC1 expression can provide useful clinical information on various cancers and neurodegenerative diseases. In order to realize the clinical applications, it is necessary to establish a stable supply of antibody and reproducible procedures for the laboratory examinations. In the present study, we have generated mouse monoclonal antibodies for RB1CC1, and four kinds of antibodies (N1-8, N1-216, N3-2, and N3-42) were found to be optimal for clinical applications such as ELISA and immunoblots and work as well as the pre-existing polyclonal antibodies. N1-8 monoclonal antibody provided the best recognition of RB1CC1 in the clinico-pathological examination of formalin-fixed paraffin-embedded tissues. These monoclonal antibodies will help to generate new opportunities in scientific examinations in biology and clinical medicine.
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spelling pubmed-32915652012-03-06 Preparation of Mouse Monoclonal Antibody for RB1CC1 and Its Clinical Application Hama, Yusuke Chano, Tokuhiro Inui, Takuma Matsumoto, Kyoichi Okabe, Hidetoshi PLoS One Research Article RB1-inducible coiled-coil 1 (RB1CC1; also known as FIP200) plays important roles in several biological pathways such as cell proliferation and autophagy. Evaluation of RB1CC1 expression can provide useful clinical information on various cancers and neurodegenerative diseases. In order to realize the clinical applications, it is necessary to establish a stable supply of antibody and reproducible procedures for the laboratory examinations. In the present study, we have generated mouse monoclonal antibodies for RB1CC1, and four kinds of antibodies (N1-8, N1-216, N3-2, and N3-42) were found to be optimal for clinical applications such as ELISA and immunoblots and work as well as the pre-existing polyclonal antibodies. N1-8 monoclonal antibody provided the best recognition of RB1CC1 in the clinico-pathological examination of formalin-fixed paraffin-embedded tissues. These monoclonal antibodies will help to generate new opportunities in scientific examinations in biology and clinical medicine. Public Library of Science 2012-03-01 /pmc/articles/PMC3291565/ /pubmed/22396748 http://dx.doi.org/10.1371/journal.pone.0032052 Text en Hama et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hama, Yusuke
Chano, Tokuhiro
Inui, Takuma
Matsumoto, Kyoichi
Okabe, Hidetoshi
Preparation of Mouse Monoclonal Antibody for RB1CC1 and Its Clinical Application
title Preparation of Mouse Monoclonal Antibody for RB1CC1 and Its Clinical Application
title_full Preparation of Mouse Monoclonal Antibody for RB1CC1 and Its Clinical Application
title_fullStr Preparation of Mouse Monoclonal Antibody for RB1CC1 and Its Clinical Application
title_full_unstemmed Preparation of Mouse Monoclonal Antibody for RB1CC1 and Its Clinical Application
title_short Preparation of Mouse Monoclonal Antibody for RB1CC1 and Its Clinical Application
title_sort preparation of mouse monoclonal antibody for rb1cc1 and its clinical application
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291565/
https://www.ncbi.nlm.nih.gov/pubmed/22396748
http://dx.doi.org/10.1371/journal.pone.0032052
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