Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging

Lipid droplets (LDs) are dynamic cellular organelles responsible for the storage of neutral lipids, and are associated with a multitude of metabolic syndromes. Here we report monodansylpentane (MDH) as a high contrast blue-fluorescent marker for LDs. The unique spectral properties make MDH easily co...

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Detalles Bibliográficos
Autores principales: Yang, Huei-Jiun, Hsu, Chia-Ling, Yang, Jin-Yi, Yang, Wei Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291611/
https://www.ncbi.nlm.nih.gov/pubmed/22396789
http://dx.doi.org/10.1371/journal.pone.0032693
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author Yang, Huei-Jiun
Hsu, Chia-Ling
Yang, Jin-Yi
Yang, Wei Yuan
author_facet Yang, Huei-Jiun
Hsu, Chia-Ling
Yang, Jin-Yi
Yang, Wei Yuan
author_sort Yang, Huei-Jiun
collection PubMed
description Lipid droplets (LDs) are dynamic cellular organelles responsible for the storage of neutral lipids, and are associated with a multitude of metabolic syndromes. Here we report monodansylpentane (MDH) as a high contrast blue-fluorescent marker for LDs. The unique spectral properties make MDH easily combinable with other green and red fluorescent reporters for multicolor fluorescence imaging. MDH staining does not apparently affect LD trafficking, and the dye is extraordinarily photo-stable. Taken together MDH represents a reliable tool to use for the investigation of dynamic LD regulation within living cells using fluorescence microscopy.
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spelling pubmed-32916112012-03-06 Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging Yang, Huei-Jiun Hsu, Chia-Ling Yang, Jin-Yi Yang, Wei Yuan PLoS One Research Article Lipid droplets (LDs) are dynamic cellular organelles responsible for the storage of neutral lipids, and are associated with a multitude of metabolic syndromes. Here we report monodansylpentane (MDH) as a high contrast blue-fluorescent marker for LDs. The unique spectral properties make MDH easily combinable with other green and red fluorescent reporters for multicolor fluorescence imaging. MDH staining does not apparently affect LD trafficking, and the dye is extraordinarily photo-stable. Taken together MDH represents a reliable tool to use for the investigation of dynamic LD regulation within living cells using fluorescence microscopy. Public Library of Science 2012-03-01 /pmc/articles/PMC3291611/ /pubmed/22396789 http://dx.doi.org/10.1371/journal.pone.0032693 Text en Yang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yang, Huei-Jiun
Hsu, Chia-Ling
Yang, Jin-Yi
Yang, Wei Yuan
Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
title Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
title_full Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
title_fullStr Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
title_full_unstemmed Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
title_short Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
title_sort monodansylpentane as a blue-fluorescent lipid-droplet marker for multi-color live-cell imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291611/
https://www.ncbi.nlm.nih.gov/pubmed/22396789
http://dx.doi.org/10.1371/journal.pone.0032693
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