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A Portable and Autonomous Magnetic Detection Platform for Biosensing

This paper presents a prototype of a platform for biomolecular recognition detection. The system is based on a magnetoresistive biochip that performs biorecognition assays by detecting magnetically tagged targets. All the electronic circuitry for addressing, driving and reading out signals from spin...

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Autores principales: Germano, José, Martins, Verónica C., Cardoso, Filipe A., Almeida, Teresa M., Sousa, Leonel, Freitas, Paulo P., Piedade, Moisés S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291901/
https://www.ncbi.nlm.nih.gov/pubmed/22408516
http://dx.doi.org/10.3390/s90604119
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author Germano, José
Martins, Verónica C.
Cardoso, Filipe A.
Almeida, Teresa M.
Sousa, Leonel
Freitas, Paulo P.
Piedade, Moisés S.
author_facet Germano, José
Martins, Verónica C.
Cardoso, Filipe A.
Almeida, Teresa M.
Sousa, Leonel
Freitas, Paulo P.
Piedade, Moisés S.
author_sort Germano, José
collection PubMed
description This paper presents a prototype of a platform for biomolecular recognition detection. The system is based on a magnetoresistive biochip that performs biorecognition assays by detecting magnetically tagged targets. All the electronic circuitry for addressing, driving and reading out signals from spin-valve or magnetic tunnel junctions sensors is implemented using off-the-shelf components. Taking advantage of digital signal processing techniques, the acquired signals are processed in real time and transmitted to a digital analyzer that enables the user to control and follow the experiment through a graphical user interface. The developed platform is portable and capable of operating autonomously for nearly eight hours. Experimental results show that the noise level of the described platform is one order of magnitude lower than the one presented by the previously used measurement set-up. Experimental results also show that this device is able to detect magnetic nanoparticles with a diameter of 250 nm at a concentration of about 40 fM. Finally, the biomolecular recognition detection capabilities of the platform are demonstrated by performing a hybridization assay using complementary and non-complementary probes and a magnetically tagged 20mer single stranded DNA target.
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spelling pubmed-32919012012-03-09 A Portable and Autonomous Magnetic Detection Platform for Biosensing Germano, José Martins, Verónica C. Cardoso, Filipe A. Almeida, Teresa M. Sousa, Leonel Freitas, Paulo P. Piedade, Moisés S. Sensors (Basel) Article This paper presents a prototype of a platform for biomolecular recognition detection. The system is based on a magnetoresistive biochip that performs biorecognition assays by detecting magnetically tagged targets. All the electronic circuitry for addressing, driving and reading out signals from spin-valve or magnetic tunnel junctions sensors is implemented using off-the-shelf components. Taking advantage of digital signal processing techniques, the acquired signals are processed in real time and transmitted to a digital analyzer that enables the user to control and follow the experiment through a graphical user interface. The developed platform is portable and capable of operating autonomously for nearly eight hours. Experimental results show that the noise level of the described platform is one order of magnitude lower than the one presented by the previously used measurement set-up. Experimental results also show that this device is able to detect magnetic nanoparticles with a diameter of 250 nm at a concentration of about 40 fM. Finally, the biomolecular recognition detection capabilities of the platform are demonstrated by performing a hybridization assay using complementary and non-complementary probes and a magnetically tagged 20mer single stranded DNA target. Molecular Diversity Preservation International (MDPI) 2009-05-27 /pmc/articles/PMC3291901/ /pubmed/22408516 http://dx.doi.org/10.3390/s90604119 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Germano, José
Martins, Verónica C.
Cardoso, Filipe A.
Almeida, Teresa M.
Sousa, Leonel
Freitas, Paulo P.
Piedade, Moisés S.
A Portable and Autonomous Magnetic Detection Platform for Biosensing
title A Portable and Autonomous Magnetic Detection Platform for Biosensing
title_full A Portable and Autonomous Magnetic Detection Platform for Biosensing
title_fullStr A Portable and Autonomous Magnetic Detection Platform for Biosensing
title_full_unstemmed A Portable and Autonomous Magnetic Detection Platform for Biosensing
title_short A Portable and Autonomous Magnetic Detection Platform for Biosensing
title_sort portable and autonomous magnetic detection platform for biosensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291901/
https://www.ncbi.nlm.nih.gov/pubmed/22408516
http://dx.doi.org/10.3390/s90604119
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