Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study

Poly(glutamic acid) has been studied with a nanosecond T-jump experiment. A new experimental set-up based on the frequency-quadrupling of an 82 MHz Titanium-Sapphire laser allows rapid CD measurements to be performed. Combining time-resolved absorption and circular dichroism at 204 and 220 nm, we ar...

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Detalles Bibliográficos
Autores principales: Mendonça, Lucille, Hache, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292019/
https://www.ncbi.nlm.nih.gov/pubmed/22408450
http://dx.doi.org/10.3390/ijms13022239
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author Mendonça, Lucille
Hache, François
author_facet Mendonça, Lucille
Hache, François
author_sort Mendonça, Lucille
collection PubMed
description Poly(glutamic acid) has been studied with a nanosecond T-jump experiment. A new experimental set-up based on the frequency-quadrupling of an 82 MHz Titanium-Sapphire laser allows rapid CD measurements to be performed. Combining time-resolved absorption and circular dichroism at 204 and 220 nm, we are able to measure precisely the unfolding relaxation time as well as the helical fraction evolution. We show that only CD at 220 nm is relevant to observe the unfolding of an alpha helix whereas no change is observed for CD at 204 nm. Conversely, both absorptions yield information on the dynamics of the process.
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spelling pubmed-32920192012-03-09 Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study Mendonça, Lucille Hache, François Int J Mol Sci Article Poly(glutamic acid) has been studied with a nanosecond T-jump experiment. A new experimental set-up based on the frequency-quadrupling of an 82 MHz Titanium-Sapphire laser allows rapid CD measurements to be performed. Combining time-resolved absorption and circular dichroism at 204 and 220 nm, we are able to measure precisely the unfolding relaxation time as well as the helical fraction evolution. We show that only CD at 220 nm is relevant to observe the unfolding of an alpha helix whereas no change is observed for CD at 204 nm. Conversely, both absorptions yield information on the dynamics of the process. Molecular Diversity Preservation International (MDPI) 2012-02-17 /pmc/articles/PMC3292019/ /pubmed/22408450 http://dx.doi.org/10.3390/ijms13022239 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Mendonça, Lucille
Hache, François
Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study
title Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study
title_full Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study
title_fullStr Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study
title_full_unstemmed Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study
title_short Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study
title_sort nanosecond t-jump experiment in poly(glutamic acid): a circular dichroism study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292019/
https://www.ncbi.nlm.nih.gov/pubmed/22408450
http://dx.doi.org/10.3390/ijms13022239
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AT hachefrancois nanosecondtjumpexperimentinpolyglutamicacidacirculardichroismstudy

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