Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma

Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on IgG...

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Detalles Bibliográficos
Autores principales: Ogawa, Takehito, Aoyagi, Satoka, Miyasaka, Takehiro, Sakai, Kiyotaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292107/
https://www.ncbi.nlm.nih.gov/pubmed/22408505
http://dx.doi.org/10.3390/s91008271
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author Ogawa, Takehito
Aoyagi, Satoka
Miyasaka, Takehiro
Sakai, Kiyotaka
author_facet Ogawa, Takehito
Aoyagi, Satoka
Miyasaka, Takehiro
Sakai, Kiyotaka
author_sort Ogawa, Takehito
collection PubMed
description Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on IgG concentration ranging from 20 to 78 μg/mL in both phosphate buffer saline and bovine plasma. This method requires no separation procedure, and the reaction time is less than 15 min. A fluorescence enhancement assay by the affinity binding of fluorescence-labeled reagent is thus available for the rapid determination of biomolecules in plasma.
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spelling pubmed-32921072012-03-09 Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma Ogawa, Takehito Aoyagi, Satoka Miyasaka, Takehiro Sakai, Kiyotaka Sensors (Basel) Communication Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on IgG concentration ranging from 20 to 78 μg/mL in both phosphate buffer saline and bovine plasma. This method requires no separation procedure, and the reaction time is less than 15 min. A fluorescence enhancement assay by the affinity binding of fluorescence-labeled reagent is thus available for the rapid determination of biomolecules in plasma. Molecular Diversity Preservation International (MDPI) 2009-10-20 /pmc/articles/PMC3292107/ /pubmed/22408505 http://dx.doi.org/10.3390/s91008271 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Ogawa, Takehito
Aoyagi, Satoka
Miyasaka, Takehiro
Sakai, Kiyotaka
Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma
title Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma
title_full Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma
title_fullStr Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma
title_full_unstemmed Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma
title_short Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma
title_sort fluorescence enhancement of fluorescein isothiocyanate-labeled protein a caused by affinity binding with immunoglobulin g in bovine plasma
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292107/
https://www.ncbi.nlm.nih.gov/pubmed/22408505
http://dx.doi.org/10.3390/s91008271
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AT miyasakatakehiro fluorescenceenhancementoffluoresceinisothiocyanatelabeledproteinacausedbyaffinitybindingwithimmunoglobulinginbovineplasma
AT sakaikiyotaka fluorescenceenhancementoffluoresceinisothiocyanatelabeledproteinacausedbyaffinitybindingwithimmunoglobulinginbovineplasma

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