Serotonin-mediated modulation of Na(+)/K(+ )pump current in rat hippocampal CA1 pyramidal neurons

BACKGROUND: The aim of this study was to investigate whether serotonin (5-hydroxytryptamine, 5-HT) can modulate Na(+)/K(+ )pump in rat hippocampal CA1 pyramidal neurons. RESULTS: 5-HT (0.1, 1 mM) showed Na(+)/K(+ )pump current (Ip) densities of 0.40 ± 0.04, 0.34 ± 0.03 pA/pF contrast to 0.63 ± 0.04...

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Detalles Bibliográficos
Autores principales: Zhang, Li Nan, Su, Su Wen, Guo, Fang, Guo, Hui Cai, Shi, Xiao Lu, Li, Wen Ya, Liu, Xu, Wang, Yong Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292479/
https://www.ncbi.nlm.nih.gov/pubmed/22257758
http://dx.doi.org/10.1186/1471-2202-13-10
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author Zhang, Li Nan
Su, Su Wen
Guo, Fang
Guo, Hui Cai
Shi, Xiao Lu
Li, Wen Ya
Liu, Xu
Wang, Yong Li
author_facet Zhang, Li Nan
Su, Su Wen
Guo, Fang
Guo, Hui Cai
Shi, Xiao Lu
Li, Wen Ya
Liu, Xu
Wang, Yong Li
author_sort Zhang, Li Nan
collection PubMed
description BACKGROUND: The aim of this study was to investigate whether serotonin (5-hydroxytryptamine, 5-HT) can modulate Na(+)/K(+ )pump in rat hippocampal CA1 pyramidal neurons. RESULTS: 5-HT (0.1, 1 mM) showed Na(+)/K(+ )pump current (Ip) densities of 0.40 ± 0.04, 0.34 ± 0.03 pA/pF contrast to 0.63 ± 0.04 pA/pF of the control of 0.5 mM strophanthidin (Str), demonstrating 5-HT-induced inhibition of Ip in a dose-dependent manner in hippocampal CA1 pyramidal neurons. The effect was partly attenuated by ondasetron, a 5-HT(3 )receptor (5-HT(3)R) antagonist, not by WAY100635, a 5-HT(1A)R antagonist, while 1-(3-Chlorophenyl) biguanide hydrochloride (m-CPBG), a 5-HT(3)R specific agonist, mimicked the effect of 5-HT on Ip. CONCLUSION: 5-HT inhibits neuronal Na(+)/K(+ )pump activity via 5-HT(3)R in rat hippocampal CA1 pyramidal neurons. This discloses novel mechanisms for the function of 5-HT in learning and memory, which may be a useful target to benefit these patients with cognitive disorder.
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spelling pubmed-32924792012-03-03 Serotonin-mediated modulation of Na(+)/K(+ )pump current in rat hippocampal CA1 pyramidal neurons Zhang, Li Nan Su, Su Wen Guo, Fang Guo, Hui Cai Shi, Xiao Lu Li, Wen Ya Liu, Xu Wang, Yong Li BMC Neurosci Research Article BACKGROUND: The aim of this study was to investigate whether serotonin (5-hydroxytryptamine, 5-HT) can modulate Na(+)/K(+ )pump in rat hippocampal CA1 pyramidal neurons. RESULTS: 5-HT (0.1, 1 mM) showed Na(+)/K(+ )pump current (Ip) densities of 0.40 ± 0.04, 0.34 ± 0.03 pA/pF contrast to 0.63 ± 0.04 pA/pF of the control of 0.5 mM strophanthidin (Str), demonstrating 5-HT-induced inhibition of Ip in a dose-dependent manner in hippocampal CA1 pyramidal neurons. The effect was partly attenuated by ondasetron, a 5-HT(3 )receptor (5-HT(3)R) antagonist, not by WAY100635, a 5-HT(1A)R antagonist, while 1-(3-Chlorophenyl) biguanide hydrochloride (m-CPBG), a 5-HT(3)R specific agonist, mimicked the effect of 5-HT on Ip. CONCLUSION: 5-HT inhibits neuronal Na(+)/K(+ )pump activity via 5-HT(3)R in rat hippocampal CA1 pyramidal neurons. This discloses novel mechanisms for the function of 5-HT in learning and memory, which may be a useful target to benefit these patients with cognitive disorder. BioMed Central 2012-01-19 /pmc/articles/PMC3292479/ /pubmed/22257758 http://dx.doi.org/10.1186/1471-2202-13-10 Text en Copyright ©2012 Zhang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Li Nan
Su, Su Wen
Guo, Fang
Guo, Hui Cai
Shi, Xiao Lu
Li, Wen Ya
Liu, Xu
Wang, Yong Li
Serotonin-mediated modulation of Na(+)/K(+ )pump current in rat hippocampal CA1 pyramidal neurons
title Serotonin-mediated modulation of Na(+)/K(+ )pump current in rat hippocampal CA1 pyramidal neurons
title_full Serotonin-mediated modulation of Na(+)/K(+ )pump current in rat hippocampal CA1 pyramidal neurons
title_fullStr Serotonin-mediated modulation of Na(+)/K(+ )pump current in rat hippocampal CA1 pyramidal neurons
title_full_unstemmed Serotonin-mediated modulation of Na(+)/K(+ )pump current in rat hippocampal CA1 pyramidal neurons
title_short Serotonin-mediated modulation of Na(+)/K(+ )pump current in rat hippocampal CA1 pyramidal neurons
title_sort serotonin-mediated modulation of na(+)/k(+ )pump current in rat hippocampal ca1 pyramidal neurons
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292479/
https://www.ncbi.nlm.nih.gov/pubmed/22257758
http://dx.doi.org/10.1186/1471-2202-13-10
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