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Feeder Cells Support the Culture of Induced Pluripotent Stem Cells Even after Chemical Fixation

Chemically fixed mouse embryonic fibroblasts (MEFs), instead of live feeder cells, were applied to the maintenance of mouse induced pluripotent stem (miPS) cells. Formaldehyde and glutaraldehyde were used for chemical fixation. The chemically fixed MEF feeders maintained the pluripotency of miPS cel...

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Detalles Bibliográficos
Autores principales: Yue, Xiao-Shan, Fujishiro, Masako, Nishioka, Chieko, Arai, Takashi, Takahashi, Eiki, Gong, Jian-Sheng, Akaike, Toshihiro, Ito, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292564/
https://www.ncbi.nlm.nih.gov/pubmed/22396791
http://dx.doi.org/10.1371/journal.pone.0032707

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