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Characterization of a calcium/calmodulin-regulated SR/CAMTA gene family during tomato fruit development and ripening

BACKGROUND: Fruit ripening is a complicated development process affected by a variety of external and internal cues. It is well established that calcium treatment delays fruit ripening and senescence. However, the underlying molecular mechanisms remain unclear. RESULTS: Previous studies have shown t...

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Autores principales: Yang, Tianbao, Peng, Hui, Whitaker, Bruce D, Conway, William S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292969/
https://www.ncbi.nlm.nih.gov/pubmed/22330838
http://dx.doi.org/10.1186/1471-2229-12-19
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author Yang, Tianbao
Peng, Hui
Whitaker, Bruce D
Conway, William S
author_facet Yang, Tianbao
Peng, Hui
Whitaker, Bruce D
Conway, William S
author_sort Yang, Tianbao
collection PubMed
description BACKGROUND: Fruit ripening is a complicated development process affected by a variety of external and internal cues. It is well established that calcium treatment delays fruit ripening and senescence. However, the underlying molecular mechanisms remain unclear. RESULTS: Previous studies have shown that calcium/calmodulin-regulated SR/CAMTAs are important for modulation of disease resistance, cold sensitivity and wounding response in vegetative tissues. To study the possible roles of this gene family in fruit development and ripening, we cloned seven SR/CAMTAs, designated as SlSRs, from tomato, a model fruit-bearing crop. All seven genes encode polypeptides with a conserved DNA-binding domain and a calmodulin-binding site. Calmodulin specifically binds to the putative targeting site in a calcium-dependent manner. All SlSRs were highly yet differentially expressed during fruit development and ripening. Most notably, the expression of SlSR2 was scarcely detected at the mature green and breaker stages, two critical stages of fruit development and ripening; and SlSR3L and SlSR4 were expressed exclusively in fruit tissues. During the developmental span from 10 to 50 days post anthesis, the expression profiles of all seven SlSRs were dramatically altered in ripening mutant rin compared with wildtype fruit. By contrast, only minor alterations were noted for ripening mutant nor and Nr fruit. In addition, ethylene treatment of mature green wildtype fruit transiently stimulated expression of all SlSRs within one to two hours. CONCLUSIONS: This study indicates that SlSR expression is influenced by both the Rin-mediated developmental network and ethylene signaling. The results suggest that calcium signaling is involved in the regulation of fruit development and ripening through calcium/calmodulin/SlSR interactions.
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spelling pubmed-32929692012-03-05 Characterization of a calcium/calmodulin-regulated SR/CAMTA gene family during tomato fruit development and ripening Yang, Tianbao Peng, Hui Whitaker, Bruce D Conway, William S BMC Plant Biol Research Article BACKGROUND: Fruit ripening is a complicated development process affected by a variety of external and internal cues. It is well established that calcium treatment delays fruit ripening and senescence. However, the underlying molecular mechanisms remain unclear. RESULTS: Previous studies have shown that calcium/calmodulin-regulated SR/CAMTAs are important for modulation of disease resistance, cold sensitivity and wounding response in vegetative tissues. To study the possible roles of this gene family in fruit development and ripening, we cloned seven SR/CAMTAs, designated as SlSRs, from tomato, a model fruit-bearing crop. All seven genes encode polypeptides with a conserved DNA-binding domain and a calmodulin-binding site. Calmodulin specifically binds to the putative targeting site in a calcium-dependent manner. All SlSRs were highly yet differentially expressed during fruit development and ripening. Most notably, the expression of SlSR2 was scarcely detected at the mature green and breaker stages, two critical stages of fruit development and ripening; and SlSR3L and SlSR4 were expressed exclusively in fruit tissues. During the developmental span from 10 to 50 days post anthesis, the expression profiles of all seven SlSRs were dramatically altered in ripening mutant rin compared with wildtype fruit. By contrast, only minor alterations were noted for ripening mutant nor and Nr fruit. In addition, ethylene treatment of mature green wildtype fruit transiently stimulated expression of all SlSRs within one to two hours. CONCLUSIONS: This study indicates that SlSR expression is influenced by both the Rin-mediated developmental network and ethylene signaling. The results suggest that calcium signaling is involved in the regulation of fruit development and ripening through calcium/calmodulin/SlSR interactions. BioMed Central 2012-02-13 /pmc/articles/PMC3292969/ /pubmed/22330838 http://dx.doi.org/10.1186/1471-2229-12-19 Text en Copyright ©2012 Yang et al; BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yang, Tianbao
Peng, Hui
Whitaker, Bruce D
Conway, William S
Characterization of a calcium/calmodulin-regulated SR/CAMTA gene family during tomato fruit development and ripening
title Characterization of a calcium/calmodulin-regulated SR/CAMTA gene family during tomato fruit development and ripening
title_full Characterization of a calcium/calmodulin-regulated SR/CAMTA gene family during tomato fruit development and ripening
title_fullStr Characterization of a calcium/calmodulin-regulated SR/CAMTA gene family during tomato fruit development and ripening
title_full_unstemmed Characterization of a calcium/calmodulin-regulated SR/CAMTA gene family during tomato fruit development and ripening
title_short Characterization of a calcium/calmodulin-regulated SR/CAMTA gene family during tomato fruit development and ripening
title_sort characterization of a calcium/calmodulin-regulated sr/camta gene family during tomato fruit development and ripening
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292969/
https://www.ncbi.nlm.nih.gov/pubmed/22330838
http://dx.doi.org/10.1186/1471-2229-12-19
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