Cargando…

High IFN-gamma and TNF production by peripheral NK cells of Colombian patients with different clinical presentation of Plasmodium falciparum

BACKGROUND: In Colombia, Plasmodium falciparum infection rarely results in severe disease or mortality compared to infections in African populations. During natural infection NK cells exhibit a cytolytic effect and regulate dendritic cells, macrophages, neutrophils as well as affect antigen specific...

Descripción completa

Detalles Bibliográficos
Autores principales: Agudelo, Olga, Bueno, Julio, Villa, Andres, Maestre, Amanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292975/
https://www.ncbi.nlm.nih.gov/pubmed/22316273
http://dx.doi.org/10.1186/1475-2875-11-38
_version_ 1782225343158943744
author Agudelo, Olga
Bueno, Julio
Villa, Andres
Maestre, Amanda
author_facet Agudelo, Olga
Bueno, Julio
Villa, Andres
Maestre, Amanda
author_sort Agudelo, Olga
collection PubMed
description BACKGROUND: In Colombia, Plasmodium falciparum infection rarely results in severe disease or mortality compared to infections in African populations. During natural infection NK cells exhibit a cytolytic effect and regulate dendritic cells, macrophages, neutrophils as well as affect antigen specific T and B cell responses. To characterize the NK cells in P. falciparum infected patients of a highly endemic region of Colombia, the degree of NK proliferation and production of IFN gamma and TNF production in these cells were explored. METHODS: Seventeen patients with acute and three with severe P. falciparum malaria patients from the Northwest region of the country were recruited in the study. In addition, 20 healthy controls were included: 10 from Medellin (no-transmission area) and 10 from the Uraba region (a malaria endemic area). Immunophenotypic analysis of peripheral mononuclear cells was performed by FACS to detect total number of NK cells, subtypes and intracellular IFNγ and TNF production by NK cells in the different patient groups. RESULTS: The total mean CD56(+)/CD3(- )NK cell proportions in acute and severe malaria subjects were 9.14% (7.15%CD56(dim), 2.01%CD56(bright)) and 19.62% (16.05%CD56(dim), 3.58%CD56(bright)), respectively, in contrast to healthy controls from endemic (total mean CD56(+)/CD3(-)1.2%) and non-endemic area (total mean CD56(+)/CD3(- )0.67%). Analysis of basal IFNγ and TNF levels confirmed the CD56(bright )NK population as the main cytokine producer (p < 0.0001) in the groups affected with malaria, with the CD56(dim )NK cell exhibiting the highest potential of TNF production after stimulus in the acute malaria group. CONCLUSIONS: The results confirm the important role of not only CD56(bright )but also of CD56(dim )NK cell populations as producers of the two cytokines in malaria patients in Colombia.
format Online
Article
Text
id pubmed-3292975
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-32929752012-03-05 High IFN-gamma and TNF production by peripheral NK cells of Colombian patients with different clinical presentation of Plasmodium falciparum Agudelo, Olga Bueno, Julio Villa, Andres Maestre, Amanda Malar J Research BACKGROUND: In Colombia, Plasmodium falciparum infection rarely results in severe disease or mortality compared to infections in African populations. During natural infection NK cells exhibit a cytolytic effect and regulate dendritic cells, macrophages, neutrophils as well as affect antigen specific T and B cell responses. To characterize the NK cells in P. falciparum infected patients of a highly endemic region of Colombia, the degree of NK proliferation and production of IFN gamma and TNF production in these cells were explored. METHODS: Seventeen patients with acute and three with severe P. falciparum malaria patients from the Northwest region of the country were recruited in the study. In addition, 20 healthy controls were included: 10 from Medellin (no-transmission area) and 10 from the Uraba region (a malaria endemic area). Immunophenotypic analysis of peripheral mononuclear cells was performed by FACS to detect total number of NK cells, subtypes and intracellular IFNγ and TNF production by NK cells in the different patient groups. RESULTS: The total mean CD56(+)/CD3(- )NK cell proportions in acute and severe malaria subjects were 9.14% (7.15%CD56(dim), 2.01%CD56(bright)) and 19.62% (16.05%CD56(dim), 3.58%CD56(bright)), respectively, in contrast to healthy controls from endemic (total mean CD56(+)/CD3(-)1.2%) and non-endemic area (total mean CD56(+)/CD3(- )0.67%). Analysis of basal IFNγ and TNF levels confirmed the CD56(bright )NK population as the main cytokine producer (p < 0.0001) in the groups affected with malaria, with the CD56(dim )NK cell exhibiting the highest potential of TNF production after stimulus in the acute malaria group. CONCLUSIONS: The results confirm the important role of not only CD56(bright )but also of CD56(dim )NK cell populations as producers of the two cytokines in malaria patients in Colombia. BioMed Central 2012-02-08 /pmc/articles/PMC3292975/ /pubmed/22316273 http://dx.doi.org/10.1186/1475-2875-11-38 Text en Copyright ©2012 Agudelo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License. (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Agudelo, Olga
Bueno, Julio
Villa, Andres
Maestre, Amanda
High IFN-gamma and TNF production by peripheral NK cells of Colombian patients with different clinical presentation of Plasmodium falciparum
title High IFN-gamma and TNF production by peripheral NK cells of Colombian patients with different clinical presentation of Plasmodium falciparum
title_full High IFN-gamma and TNF production by peripheral NK cells of Colombian patients with different clinical presentation of Plasmodium falciparum
title_fullStr High IFN-gamma and TNF production by peripheral NK cells of Colombian patients with different clinical presentation of Plasmodium falciparum
title_full_unstemmed High IFN-gamma and TNF production by peripheral NK cells of Colombian patients with different clinical presentation of Plasmodium falciparum
title_short High IFN-gamma and TNF production by peripheral NK cells of Colombian patients with different clinical presentation of Plasmodium falciparum
title_sort high ifn-gamma and tnf production by peripheral nk cells of colombian patients with different clinical presentation of plasmodium falciparum
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292975/
https://www.ncbi.nlm.nih.gov/pubmed/22316273
http://dx.doi.org/10.1186/1475-2875-11-38
work_keys_str_mv AT agudeloolga highifngammaandtnfproductionbyperipheralnkcellsofcolombianpatientswithdifferentclinicalpresentationofplasmodiumfalciparum
AT buenojulio highifngammaandtnfproductionbyperipheralnkcellsofcolombianpatientswithdifferentclinicalpresentationofplasmodiumfalciparum
AT villaandres highifngammaandtnfproductionbyperipheralnkcellsofcolombianpatientswithdifferentclinicalpresentationofplasmodiumfalciparum
AT maestreamanda highifngammaandtnfproductionbyperipheralnkcellsofcolombianpatientswithdifferentclinicalpresentationofplasmodiumfalciparum