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Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora

Three cytochrome P450 monooxygenase (CYP) genes, designated pb-1, pb-2 and pb-3, were isolated from the white-rot fungus, Phlebia brevispora, using reverse transcription PCR with degenerate primers constructed based on the consensus amino acid sequence of eukaryotic CYPs in the O(2)-binding, meander...

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Autores principales: Nakamura, Ryoich, Kondo, Ryuichiro, Shen, Ming-hao, Ochiai, Hideharu, Hisamatsu, Shin, Sonoki, Shigenori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2012
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292997/
https://www.ncbi.nlm.nih.gov/pubmed/22273259
http://dx.doi.org/10.1186/2191-0855-2-8
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author Nakamura, Ryoich
Kondo, Ryuichiro
Shen, Ming-hao
Ochiai, Hideharu
Hisamatsu, Shin
Sonoki, Shigenori
author_facet Nakamura, Ryoich
Kondo, Ryuichiro
Shen, Ming-hao
Ochiai, Hideharu
Hisamatsu, Shin
Sonoki, Shigenori
author_sort Nakamura, Ryoich
collection PubMed
description Three cytochrome P450 monooxygenase (CYP) genes, designated pb-1, pb-2 and pb-3, were isolated from the white-rot fungus, Phlebia brevispora, using reverse transcription PCR with degenerate primers constructed based on the consensus amino acid sequence of eukaryotic CYPs in the O(2)-binding, meander and heme-binding regions. Individual full-length CYP cDNAs were cloned and sequenced, and the relative nucleotide sequence similarity of pb-1 (1788 bp), pb-2 (1881 bp) and pb-3 (1791 bp) was more than 58%. Alignment of the deduced amino acid (aa) sequences of pb-1-pb-3 showed that these three CYPs belong to the same family with > 40% aa sequence similarity, and pb-1 and pb-3 are in the same subfamily, with > 55% aa sequence similarity. Furthermore, pb-1-pb-3 appeared to be a subfamily of CYP63A (CYP63A1-CYP63A4), found in Phanerochaete chrysosporium. The phylogenetic tree constructed by 500 bootstrap replications using the neighbor-joining method showed that the evolutionary distance between pb-1 and pb-3 was shorter than that between pb-2 and pb-1 (or pb-3). Exon-intron analysis of pb-1 and pb-3 showed that both genes have nearly the same number, size and order of exons and the types of introns, also indicating both genes appear to be evolutionarily close. It is interesting that the transcription level of pb-3 was evidently increased above the pb-1 transcription level by exposure to 12 coplanar PCB congeners and 2,3,7,8-tetrachlorodibenzo-p-dioxin, though the two genes were evolutionarily close.
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spelling pubmed-32929972012-03-06 Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora Nakamura, Ryoich Kondo, Ryuichiro Shen, Ming-hao Ochiai, Hideharu Hisamatsu, Shin Sonoki, Shigenori AMB Express Original Three cytochrome P450 monooxygenase (CYP) genes, designated pb-1, pb-2 and pb-3, were isolated from the white-rot fungus, Phlebia brevispora, using reverse transcription PCR with degenerate primers constructed based on the consensus amino acid sequence of eukaryotic CYPs in the O(2)-binding, meander and heme-binding regions. Individual full-length CYP cDNAs were cloned and sequenced, and the relative nucleotide sequence similarity of pb-1 (1788 bp), pb-2 (1881 bp) and pb-3 (1791 bp) was more than 58%. Alignment of the deduced amino acid (aa) sequences of pb-1-pb-3 showed that these three CYPs belong to the same family with > 40% aa sequence similarity, and pb-1 and pb-3 are in the same subfamily, with > 55% aa sequence similarity. Furthermore, pb-1-pb-3 appeared to be a subfamily of CYP63A (CYP63A1-CYP63A4), found in Phanerochaete chrysosporium. The phylogenetic tree constructed by 500 bootstrap replications using the neighbor-joining method showed that the evolutionary distance between pb-1 and pb-3 was shorter than that between pb-2 and pb-1 (or pb-3). Exon-intron analysis of pb-1 and pb-3 showed that both genes have nearly the same number, size and order of exons and the types of introns, also indicating both genes appear to be evolutionarily close. It is interesting that the transcription level of pb-3 was evidently increased above the pb-1 transcription level by exposure to 12 coplanar PCB congeners and 2,3,7,8-tetrachlorodibenzo-p-dioxin, though the two genes were evolutionarily close. Springer 2012-01-25 /pmc/articles/PMC3292997/ /pubmed/22273259 http://dx.doi.org/10.1186/2191-0855-2-8 Text en Copyright ©2012 Nakamura et al; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original
Nakamura, Ryoich
Kondo, Ryuichiro
Shen, Ming-hao
Ochiai, Hideharu
Hisamatsu, Shin
Sonoki, Shigenori
Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora
title Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora
title_full Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora
title_fullStr Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora
title_full_unstemmed Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora
title_short Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora
title_sort identification of cytochrome p450 monooxygenase genes from the white-rot fungus phlebia brevispora
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292997/
https://www.ncbi.nlm.nih.gov/pubmed/22273259
http://dx.doi.org/10.1186/2191-0855-2-8
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