An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence sig...

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Detalles Bibliográficos
Autores principales: Hasenkamp, Sandra, Wong, Eleanor H, Horrocks, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293040/
https://www.ncbi.nlm.nih.gov/pubmed/22325061
http://dx.doi.org/10.1186/1475-2875-11-42
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author Hasenkamp, Sandra
Wong, Eleanor H
Horrocks, Paul
author_facet Hasenkamp, Sandra
Wong, Eleanor H
Horrocks, Paul
author_sort Hasenkamp, Sandra
collection PubMed
description This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.
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spelling pubmed-32930402012-03-05 An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum Hasenkamp, Sandra Wong, Eleanor H Horrocks, Paul Malar J Methodology This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity. BioMed Central 2012-02-10 /pmc/articles/PMC3293040/ /pubmed/22325061 http://dx.doi.org/10.1186/1475-2875-11-42 Text en Copyright ©2012 Hasenkamp et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Hasenkamp, Sandra
Wong, Eleanor H
Horrocks, Paul
An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
title An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
title_full An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
title_fullStr An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
title_full_unstemmed An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
title_short An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
title_sort improved single-step lysis protocol to measure luciferase bioluminescence in plasmodium falciparum
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293040/
https://www.ncbi.nlm.nih.gov/pubmed/22325061
http://dx.doi.org/10.1186/1475-2875-11-42
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