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An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence sig...

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Detalles Bibliográficos
Autores principales: Hasenkamp, Sandra, Wong, Eleanor H, Horrocks, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293040/
https://www.ncbi.nlm.nih.gov/pubmed/22325061
http://dx.doi.org/10.1186/1475-2875-11-42