A method for non-invasive genotyping of APC(min/+ )mice using fecal samples

The APC(min/+ )mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reprod...

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Detalles Bibliográficos
Autores principales: Symonds, Erin L, Fenech, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293049/
https://www.ncbi.nlm.nih.gov/pubmed/22284906
http://dx.doi.org/10.1186/1480-9222-14-1
Descripción
Sumario:The APC(min/+ )mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reproducible method for genotyping mice with DNA extracted from fecal samples. Comparison of fecal results with those obtained from intestinal tissue DNA and clinical outcome (presence/absence of tumors) showed this technique to have 100% accuracy. This non-invasive method of genotyping may be applied to other transgenic mouse models.

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