A method for non-invasive genotyping of APC(min/+ )mice using fecal samples

The APC(min/+ )mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reprod...

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Detalles Bibliográficos
Autores principales: Symonds, Erin L, Fenech, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293049/
https://www.ncbi.nlm.nih.gov/pubmed/22284906
http://dx.doi.org/10.1186/1480-9222-14-1
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author Symonds, Erin L
Fenech, Michael
author_facet Symonds, Erin L
Fenech, Michael
author_sort Symonds, Erin L
collection PubMed
description The APC(min/+ )mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reproducible method for genotyping mice with DNA extracted from fecal samples. Comparison of fecal results with those obtained from intestinal tissue DNA and clinical outcome (presence/absence of tumors) showed this technique to have 100% accuracy. This non-invasive method of genotyping may be applied to other transgenic mouse models.
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spelling pubmed-32930492012-03-05 A method for non-invasive genotyping of APC(min/+ )mice using fecal samples Symonds, Erin L Fenech, Michael Biol Proced Online Methodology The APC(min/+ )mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reproducible method for genotyping mice with DNA extracted from fecal samples. Comparison of fecal results with those obtained from intestinal tissue DNA and clinical outcome (presence/absence of tumors) showed this technique to have 100% accuracy. This non-invasive method of genotyping may be applied to other transgenic mouse models. BioMed Central 2012-01-30 /pmc/articles/PMC3293049/ /pubmed/22284906 http://dx.doi.org/10.1186/1480-9222-14-1 Text en Copyright ©2012 Symonds and Fenech; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Symonds, Erin L
Fenech, Michael
A method for non-invasive genotyping of APC(min/+ )mice using fecal samples
title A method for non-invasive genotyping of APC(min/+ )mice using fecal samples
title_full A method for non-invasive genotyping of APC(min/+ )mice using fecal samples
title_fullStr A method for non-invasive genotyping of APC(min/+ )mice using fecal samples
title_full_unstemmed A method for non-invasive genotyping of APC(min/+ )mice using fecal samples
title_short A method for non-invasive genotyping of APC(min/+ )mice using fecal samples
title_sort method for non-invasive genotyping of apc(min/+ )mice using fecal samples
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293049/
https://www.ncbi.nlm.nih.gov/pubmed/22284906
http://dx.doi.org/10.1186/1480-9222-14-1
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