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Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana

The potential use of algae in biofuels applications is receiving significant attention. However, none of the current algal model species are competitive production strains. Here we present a draft genome sequence and a genetic transformation method for the marine microalga Nannochloropsis gaditana C...

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Autores principales: Radakovits, Randor, Jinkerson, Robert E., Fuerstenberg, Susan I., Tae, Hongseok, Settlage, Robert E., Boore, Jeffrey L., Posewitz, Matthew C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293424/
https://www.ncbi.nlm.nih.gov/pubmed/22353717
http://dx.doi.org/10.1038/ncomms1688
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author Radakovits, Randor
Jinkerson, Robert E.
Fuerstenberg, Susan I.
Tae, Hongseok
Settlage, Robert E.
Boore, Jeffrey L.
Posewitz, Matthew C.
author_facet Radakovits, Randor
Jinkerson, Robert E.
Fuerstenberg, Susan I.
Tae, Hongseok
Settlage, Robert E.
Boore, Jeffrey L.
Posewitz, Matthew C.
author_sort Radakovits, Randor
collection PubMed
description The potential use of algae in biofuels applications is receiving significant attention. However, none of the current algal model species are competitive production strains. Here we present a draft genome sequence and a genetic transformation method for the marine microalga Nannochloropsis gaditana CCMP526. We show that N. gaditana has highly favourable lipid yields, and is a promising production organism. The genome assembly includes nuclear (~29 Mb) and organellar genomes, and contains 9,052 gene models. We define the genes required for glycerolipid biogenesis and detail the differential regulation of genes during nitrogen-limited lipid biosynthesis. Phylogenomic analysis identifies genetic attributes of this organism, including unique stramenopile photosynthesis genes and gene expansions that may explain the distinguishing photoautotrophic phenotypes observed. The availability of a genome sequence and transformation methods will facilitate investigations into N. gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga.
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spelling pubmed-32934242012-03-05 Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana Radakovits, Randor Jinkerson, Robert E. Fuerstenberg, Susan I. Tae, Hongseok Settlage, Robert E. Boore, Jeffrey L. Posewitz, Matthew C. Nat Commun Article The potential use of algae in biofuels applications is receiving significant attention. However, none of the current algal model species are competitive production strains. Here we present a draft genome sequence and a genetic transformation method for the marine microalga Nannochloropsis gaditana CCMP526. We show that N. gaditana has highly favourable lipid yields, and is a promising production organism. The genome assembly includes nuclear (~29 Mb) and organellar genomes, and contains 9,052 gene models. We define the genes required for glycerolipid biogenesis and detail the differential regulation of genes during nitrogen-limited lipid biosynthesis. Phylogenomic analysis identifies genetic attributes of this organism, including unique stramenopile photosynthesis genes and gene expansions that may explain the distinguishing photoautotrophic phenotypes observed. The availability of a genome sequence and transformation methods will facilitate investigations into N. gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga. Nature Pub. Group 2012-02-21 /pmc/articles/PMC3293424/ /pubmed/22353717 http://dx.doi.org/10.1038/ncomms1688 Text en Copyright © 2012, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Radakovits, Randor
Jinkerson, Robert E.
Fuerstenberg, Susan I.
Tae, Hongseok
Settlage, Robert E.
Boore, Jeffrey L.
Posewitz, Matthew C.
Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana
title Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana
title_full Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana
title_fullStr Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana
title_full_unstemmed Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana
title_short Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana
title_sort draft genome sequence and genetic transformation of the oleaginous alga nannochloropis gaditana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293424/
https://www.ncbi.nlm.nih.gov/pubmed/22353717
http://dx.doi.org/10.1038/ncomms1688
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