Generation of Full-Length cDNAs for Eight Putative GPCnR from the Cattle Tick, R. microplus Using a Targeted Degenerate PCR and Sequencing Strategy

We describe here a rapid and efficient method for the targeted isolation of specific members of gene families without the need for cloning. Using this strategy we isolated full length cDNAs for eight putative G-protein coupled neurotransmitter receptors (GPCnR) from the cattle tick Rhipicephalus (Bo...

Descripción completa

Detalles Bibliográficos
Autores principales: Corley, Sean W., Piper, Emily K., Jonsson, Nicholas N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293813/
https://www.ncbi.nlm.nih.gov/pubmed/22403662
http://dx.doi.org/10.1371/journal.pone.0032480

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293813/
https://www.ncbi.nlm.nih.gov/pubmed/22403662
http://dx.doi.org/10.1371/journal.pone.0032480

Ejemplares similares