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MotifMap: integrative genome-wide maps of regulatory motif sites for model species
BACKGROUND: A central challenge of biology is to map and understand gene regulation on a genome-wide scale. For any given genome, only a small fraction of the regulatory elements embedded in the DNA sequence have been characterized, and there is great interest in developing computational methods to...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293935/ https://www.ncbi.nlm.nih.gov/pubmed/22208852 http://dx.doi.org/10.1186/1471-2105-12-495 |
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author | Daily, Kenneth Patel, Vishal R Rigor, Paul Xie, Xiaohui Baldi, Pierre |
author_facet | Daily, Kenneth Patel, Vishal R Rigor, Paul Xie, Xiaohui Baldi, Pierre |
author_sort | Daily, Kenneth |
collection | PubMed |
description | BACKGROUND: A central challenge of biology is to map and understand gene regulation on a genome-wide scale. For any given genome, only a small fraction of the regulatory elements embedded in the DNA sequence have been characterized, and there is great interest in developing computational methods to systematically map all these elements and understand their relationships. Such computational efforts, however, are significantly hindered by the overwhelming size of non-coding regions and the statistical variability and complex spatial organizations of regulatory elements and interactions. Genome-wide catalogs of regulatory elements for all model species simply do not yet exist. RESULTS: The MotifMap system uses databases of transcription factor binding motifs, refined genome alignments, and a comparative genomic statistical approach to provide comprehensive maps of candidate regulatory elements encoded in the genomes of model species. The system is used to derive new genome-wide maps for yeast, fly, worm, mouse, and human. The human map contains 519,108 sites for 570 matrices with a False Discovery Rate of 0.1 or less. The new maps are assessed in several ways, for instance using high-throughput experimental ChIP-seq data and AUC statistics, providing strong evidence for their accuracy and coverage. The maps can be usefully integrated with many other kinds of omic data and are available at http://motifmap.igb.uci.edu/. CONCLUSIONS: MotifMap and its integration with other data provide a foundation for analyzing gene regulation on a genome-wide scale, and for automatically generating regulatory pathways and hypotheses. The power of this approach is demonstrated and discussed using the P53 apoptotic pathway and the Gli hedgehog pathways as examples. |
format | Online Article Text |
id | pubmed-3293935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32939352012-03-06 MotifMap: integrative genome-wide maps of regulatory motif sites for model species Daily, Kenneth Patel, Vishal R Rigor, Paul Xie, Xiaohui Baldi, Pierre BMC Bioinformatics Research Article BACKGROUND: A central challenge of biology is to map and understand gene regulation on a genome-wide scale. For any given genome, only a small fraction of the regulatory elements embedded in the DNA sequence have been characterized, and there is great interest in developing computational methods to systematically map all these elements and understand their relationships. Such computational efforts, however, are significantly hindered by the overwhelming size of non-coding regions and the statistical variability and complex spatial organizations of regulatory elements and interactions. Genome-wide catalogs of regulatory elements for all model species simply do not yet exist. RESULTS: The MotifMap system uses databases of transcription factor binding motifs, refined genome alignments, and a comparative genomic statistical approach to provide comprehensive maps of candidate regulatory elements encoded in the genomes of model species. The system is used to derive new genome-wide maps for yeast, fly, worm, mouse, and human. The human map contains 519,108 sites for 570 matrices with a False Discovery Rate of 0.1 or less. The new maps are assessed in several ways, for instance using high-throughput experimental ChIP-seq data and AUC statistics, providing strong evidence for their accuracy and coverage. The maps can be usefully integrated with many other kinds of omic data and are available at http://motifmap.igb.uci.edu/. CONCLUSIONS: MotifMap and its integration with other data provide a foundation for analyzing gene regulation on a genome-wide scale, and for automatically generating regulatory pathways and hypotheses. The power of this approach is demonstrated and discussed using the P53 apoptotic pathway and the Gli hedgehog pathways as examples. BioMed Central 2011-12-30 /pmc/articles/PMC3293935/ /pubmed/22208852 http://dx.doi.org/10.1186/1471-2105-12-495 Text en Copyright ©2011 Daily et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Daily, Kenneth Patel, Vishal R Rigor, Paul Xie, Xiaohui Baldi, Pierre MotifMap: integrative genome-wide maps of regulatory motif sites for model species |
title | MotifMap: integrative genome-wide maps of regulatory motif sites for model species |
title_full | MotifMap: integrative genome-wide maps of regulatory motif sites for model species |
title_fullStr | MotifMap: integrative genome-wide maps of regulatory motif sites for model species |
title_full_unstemmed | MotifMap: integrative genome-wide maps of regulatory motif sites for model species |
title_short | MotifMap: integrative genome-wide maps of regulatory motif sites for model species |
title_sort | motifmap: integrative genome-wide maps of regulatory motif sites for model species |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293935/ https://www.ncbi.nlm.nih.gov/pubmed/22208852 http://dx.doi.org/10.1186/1471-2105-12-495 |
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