Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells

Microarray gene-expression profiles are generally validated one gene at a time by real-time RT-PCR. We describe here a different approach based on simultaneous mutual validation of large numbers of genes using two different expression-profiling platforms. The result described here for the NCI-60 can...

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Detalles Bibliográficos
Autores principales: Lee, Jae K, Bussey, Kimberly J, Gwadry, Fuad G, Reinhold, William, Riddick, Gregory, Pelletier, Sandra L, Nishizuka, Satoshi, Szakacs, Gergely, Annereau, Jean-Phillipe, Shankavaram, Uma, Lababidi, Samir, Smith, Lawrence H, Gottesman, Michael M, Weinstein, John N
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC329421/
https://www.ncbi.nlm.nih.gov/pubmed/14659019
Descripción
Sumario:Microarray gene-expression profiles are generally validated one gene at a time by real-time RT-PCR. We describe here a different approach based on simultaneous mutual validation of large numbers of genes using two different expression-profiling platforms. The result described here for the NCI-60 cancer cell lines is a consensus set of genes that give similar profiles on spotted cDNA arrays and Affymetrix oligonucleotide chips. Global concordance is parameterized by a 'correlation of correlations' coefficient.

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