Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells

Microarray gene-expression profiles are generally validated one gene at a time by real-time RT-PCR. We describe here a different approach based on simultaneous mutual validation of large numbers of genes using two different expression-profiling platforms. The result described here for the NCI-60 can...

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Autores principales: Lee, Jae K, Bussey, Kimberly J, Gwadry, Fuad G, Reinhold, William, Riddick, Gregory, Pelletier, Sandra L, Nishizuka, Satoshi, Szakacs, Gergely, Annereau, Jean-Phillipe, Shankavaram, Uma, Lababidi, Samir, Smith, Lawrence H, Gottesman, Michael M, Weinstein, John N
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC329421/
https://www.ncbi.nlm.nih.gov/pubmed/14659019
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author Lee, Jae K
Bussey, Kimberly J
Gwadry, Fuad G
Reinhold, William
Riddick, Gregory
Pelletier, Sandra L
Nishizuka, Satoshi
Szakacs, Gergely
Annereau, Jean-Phillipe
Shankavaram, Uma
Lababidi, Samir
Smith, Lawrence H
Gottesman, Michael M
Weinstein, John N
author_facet Lee, Jae K
Bussey, Kimberly J
Gwadry, Fuad G
Reinhold, William
Riddick, Gregory
Pelletier, Sandra L
Nishizuka, Satoshi
Szakacs, Gergely
Annereau, Jean-Phillipe
Shankavaram, Uma
Lababidi, Samir
Smith, Lawrence H
Gottesman, Michael M
Weinstein, John N
author_sort Lee, Jae K
collection PubMed
description Microarray gene-expression profiles are generally validated one gene at a time by real-time RT-PCR. We describe here a different approach based on simultaneous mutual validation of large numbers of genes using two different expression-profiling platforms. The result described here for the NCI-60 cancer cell lines is a consensus set of genes that give similar profiles on spotted cDNA arrays and Affymetrix oligonucleotide chips. Global concordance is parameterized by a 'correlation of correlations' coefficient.
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spelling pubmed-3294212004-02-05 Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells Lee, Jae K Bussey, Kimberly J Gwadry, Fuad G Reinhold, William Riddick, Gregory Pelletier, Sandra L Nishizuka, Satoshi Szakacs, Gergely Annereau, Jean-Phillipe Shankavaram, Uma Lababidi, Samir Smith, Lawrence H Gottesman, Michael M Weinstein, John N Genome Biol Method Microarray gene-expression profiles are generally validated one gene at a time by real-time RT-PCR. We describe here a different approach based on simultaneous mutual validation of large numbers of genes using two different expression-profiling platforms. The result described here for the NCI-60 cancer cell lines is a consensus set of genes that give similar profiles on spotted cDNA arrays and Affymetrix oligonucleotide chips. Global concordance is parameterized by a 'correlation of correlations' coefficient. BioMed Central 2003 2003-11-25 /pmc/articles/PMC329421/ /pubmed/14659019 Text en Copyright © 2003 Lee et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Method
Lee, Jae K
Bussey, Kimberly J
Gwadry, Fuad G
Reinhold, William
Riddick, Gregory
Pelletier, Sandra L
Nishizuka, Satoshi
Szakacs, Gergely
Annereau, Jean-Phillipe
Shankavaram, Uma
Lababidi, Samir
Smith, Lawrence H
Gottesman, Michael M
Weinstein, John N
Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells
title Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells
title_full Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells
title_fullStr Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells
title_full_unstemmed Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells
title_short Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells
title_sort comparing cdna and oligonucleotide array data: concordance of gene expression across platforms for the nci-60 cancer cells
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC329421/
https://www.ncbi.nlm.nih.gov/pubmed/14659019
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