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Deep Sequencing Analyses of Low Density Microbial Communities: Working at the Boundary of Accurate Microbiota Detection
INTRODUCTION: Accurate analyses of microbiota composition of low-density communities (10(3)–10(4) bacteria/sample) can be challenging. Background DNA from chemicals and consumables, extraction biases as well as differences in PCR efficiency can significantly interfere with microbiota assessment. Thi...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3295791/ https://www.ncbi.nlm.nih.gov/pubmed/22412957 http://dx.doi.org/10.1371/journal.pone.0032942 |