Cargando…
Assigning a function to a conserved archaeal metallo-β-lactamase from Haloferax volcanii
The metallo-β-lactamase family of enzymes comprises a large group of proteins with diverse functions in the metabolism of the cell. Among others, this superfamily contains proteins which are involved in DNA and RNA metabolism, acting as nucleases in e.g. repair and maturation. Many proteins have bee...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Japan
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296008/ https://www.ncbi.nlm.nih.gov/pubmed/22350204 http://dx.doi.org/10.1007/s00792-012-0433-4 |
_version_ | 1782225662090674176 |
---|---|
author | Fischer, Susan John von Freyend, Simona Sabag-Daigle, Anice Daniels, Charles J. Allers, Thorsten Marchfelder, Anita |
author_facet | Fischer, Susan John von Freyend, Simona Sabag-Daigle, Anice Daniels, Charles J. Allers, Thorsten Marchfelder, Anita |
author_sort | Fischer, Susan |
collection | PubMed |
description | The metallo-β-lactamase family of enzymes comprises a large group of proteins with diverse functions in the metabolism of the cell. Among others, this superfamily contains proteins which are involved in DNA and RNA metabolism, acting as nucleases in e.g. repair and maturation. Many proteins have been annotated in prokaryotic genomes as being potential metallo-β-lactamases, but very often the function has not been proven. The protein HVO_2763 from Haloferax volcanii is such a potential metallo-β-lactamase. HVO_2763 has sequence similarity to the metallo-β-lactamase tRNase Z, a tRNA 3′ processing endonuclease. Here, we report the characterisation of this metallo-β-lactamase HVO_2763 in the halophilic archaeon Haloferax volcanii. Using different in vitro assays with the recombinant HVO_2763, we could show that the protein does not have tRNA 3′ processing or exonuclease activity. According to transcriptome analyses of the HVO_2763 deletion strain, expression of proteins involved in membrane transport is downregulated in the mutant. Therefore, HVO_2763 might be involved directly or indirectly in membrane transport. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00792-012-0433-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-3296008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-32960082012-03-21 Assigning a function to a conserved archaeal metallo-β-lactamase from Haloferax volcanii Fischer, Susan John von Freyend, Simona Sabag-Daigle, Anice Daniels, Charles J. Allers, Thorsten Marchfelder, Anita Extremophiles Original Paper The metallo-β-lactamase family of enzymes comprises a large group of proteins with diverse functions in the metabolism of the cell. Among others, this superfamily contains proteins which are involved in DNA and RNA metabolism, acting as nucleases in e.g. repair and maturation. Many proteins have been annotated in prokaryotic genomes as being potential metallo-β-lactamases, but very often the function has not been proven. The protein HVO_2763 from Haloferax volcanii is such a potential metallo-β-lactamase. HVO_2763 has sequence similarity to the metallo-β-lactamase tRNase Z, a tRNA 3′ processing endonuclease. Here, we report the characterisation of this metallo-β-lactamase HVO_2763 in the halophilic archaeon Haloferax volcanii. Using different in vitro assays with the recombinant HVO_2763, we could show that the protein does not have tRNA 3′ processing or exonuclease activity. According to transcriptome analyses of the HVO_2763 deletion strain, expression of proteins involved in membrane transport is downregulated in the mutant. Therefore, HVO_2763 might be involved directly or indirectly in membrane transport. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00792-012-0433-4) contains supplementary material, which is available to authorized users. Springer Japan 2012-02-18 2012 /pmc/articles/PMC3296008/ /pubmed/22350204 http://dx.doi.org/10.1007/s00792-012-0433-4 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Paper Fischer, Susan John von Freyend, Simona Sabag-Daigle, Anice Daniels, Charles J. Allers, Thorsten Marchfelder, Anita Assigning a function to a conserved archaeal metallo-β-lactamase from Haloferax volcanii |
title | Assigning a function to a conserved archaeal metallo-β-lactamase from Haloferax volcanii |
title_full | Assigning a function to a conserved archaeal metallo-β-lactamase from Haloferax volcanii |
title_fullStr | Assigning a function to a conserved archaeal metallo-β-lactamase from Haloferax volcanii |
title_full_unstemmed | Assigning a function to a conserved archaeal metallo-β-lactamase from Haloferax volcanii |
title_short | Assigning a function to a conserved archaeal metallo-β-lactamase from Haloferax volcanii |
title_sort | assigning a function to a conserved archaeal metallo-β-lactamase from haloferax volcanii |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296008/ https://www.ncbi.nlm.nih.gov/pubmed/22350204 http://dx.doi.org/10.1007/s00792-012-0433-4 |
work_keys_str_mv | AT fischersusan assigningafunctiontoaconservedarchaealmetalloblactamasefromhaloferaxvolcanii AT johnvonfreyendsimona assigningafunctiontoaconservedarchaealmetalloblactamasefromhaloferaxvolcanii AT sabagdaigleanice assigningafunctiontoaconservedarchaealmetalloblactamasefromhaloferaxvolcanii AT danielscharlesj assigningafunctiontoaconservedarchaealmetalloblactamasefromhaloferaxvolcanii AT allersthorsten assigningafunctiontoaconservedarchaealmetalloblactamasefromhaloferaxvolcanii AT marchfelderanita assigningafunctiontoaconservedarchaealmetalloblactamasefromhaloferaxvolcanii |