Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps

We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumi...

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Detalles Bibliográficos
Autores principales: Verchère, Alice, Broutin, Isabelle, Picard, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296093/
https://www.ncbi.nlm.nih.gov/pubmed/22400101
http://dx.doi.org/10.1038/srep00306
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author Verchère, Alice
Broutin, Isabelle
Picard, Martin
author_facet Verchère, Alice
Broutin, Isabelle
Picard, Martin
author_sort Verchère, Alice
collection PubMed
description We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumination with visible light, the photo-induced proton gradient created by the BR is shown to be coupled to the active transport of substrates through the pump.
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spelling pubmed-32960932012-03-07 Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps Verchère, Alice Broutin, Isabelle Picard, Martin Sci Rep Article We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumination with visible light, the photo-induced proton gradient created by the BR is shown to be coupled to the active transport of substrates through the pump. Nature Publishing Group 2012-03-07 /pmc/articles/PMC3296093/ /pubmed/22400101 http://dx.doi.org/10.1038/srep00306 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Verchère, Alice
Broutin, Isabelle
Picard, Martin
Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps
title Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps
title_full Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps
title_fullStr Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps
title_full_unstemmed Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps
title_short Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps
title_sort photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296093/
https://www.ncbi.nlm.nih.gov/pubmed/22400101
http://dx.doi.org/10.1038/srep00306
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