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Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps
We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumi...
Autores principales: | Verchère, Alice, Broutin, Isabelle, Picard, Martin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296093/ https://www.ncbi.nlm.nih.gov/pubmed/22400101 http://dx.doi.org/10.1038/srep00306 |
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