Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy

Immunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the l...

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Autores principales: Cason, Chevelle A., Fabré, Thomas A., Buhrlage, Andrew, Haik, Kristi L., Bullen, Heather A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296299/
https://www.ncbi.nlm.nih.gov/pubmed/22505915
http://dx.doi.org/10.1155/2012/341260
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author Cason, Chevelle A.
Fabré, Thomas A.
Buhrlage, Andrew
Haik, Kristi L.
Bullen, Heather A.
author_facet Cason, Chevelle A.
Fabré, Thomas A.
Buhrlage, Andrew
Haik, Kristi L.
Bullen, Heather A.
author_sort Cason, Chevelle A.
collection PubMed
description Immunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the low-level detection of dendrimers, improving the limit of detection by a factor of 1000 (LOD = 2.5 × 10(−13) moles). The biorecognition method is reproducible and shows high specificity and good accuracy. In addition, the capture assay platform shows a promising approach to patterning dendrimers for nanotechnology applications.
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spelling pubmed-32962992012-04-13 Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy Cason, Chevelle A. Fabré, Thomas A. Buhrlage, Andrew Haik, Kristi L. Bullen, Heather A. Int J Anal Chem Research Article Immunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the low-level detection of dendrimers, improving the limit of detection by a factor of 1000 (LOD = 2.5 × 10(−13) moles). The biorecognition method is reproducible and shows high specificity and good accuracy. In addition, the capture assay platform shows a promising approach to patterning dendrimers for nanotechnology applications. Hindawi Publishing Corporation 2012 2012-02-08 /pmc/articles/PMC3296299/ /pubmed/22505915 http://dx.doi.org/10.1155/2012/341260 Text en Copyright © 2012 Chevelle A. Cason et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cason, Chevelle A.
Fabré, Thomas A.
Buhrlage, Andrew
Haik, Kristi L.
Bullen, Heather A.
Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title_full Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title_fullStr Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title_full_unstemmed Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title_short Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title_sort low-level detection of poly(amidoamine) pamam dendrimers using immunoimaging scanning probe microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296299/
https://www.ncbi.nlm.nih.gov/pubmed/22505915
http://dx.doi.org/10.1155/2012/341260
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