Cargando…

Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract

Matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry was used for the confirmation of hapten number in synthesized antigen. As application of MAb, the MAbs against ginsenosides and glycyrrhizin have been prepared resulting in the development of two new techniques that we named t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tanaka, Hiroyuki, Morinaga, Osamu, Uto, Takuhiro, Fuji, Shunsuke, Aboagye, Frederick Asare, Tung, Nguyen Huu, Li, Xiao Wei, Putalun, Waraporn, Shoyama, Yukihiro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2012
Materias:	Review Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296308/ https://www.ncbi.nlm.nih.gov/pubmed/22518137 http://dx.doi.org/10.1155/2012/260425

Ejemplares similares

Monoclonal Antibodies against Small Molecule Natural Products and Their Applications, Eastern Blotting and Knockout Extract
por: Shoyama, Yukihiro
Publicado: (2011)

Pharmacological Effects of Ginseng on Liver Functions and Diseases: A Minireview
por: Huu Tung, Nguyen, et al.
Publicado: (2012)

Hirsutanone Isolated from the Bark of Alnus japonica Attenuates Melanogenesis via Dual Inhibition of Tyrosinase Activity and Expression of Melanogenic Proteins
por: Uto, Takuhiro, et al.
Publicado: (2022)

Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies
por: Uto, Takuhiro, et al.
Publicado: (2021)

Molecular Mechanisms Underlying Anti-Inflammatory Actions of 6-(Methylsulfinyl)hexyl Isothiocyanate Derived from Wasabi (Wasabia japonica)
por: Uto, Takuhiro, et al.
Publicado: (2012)

Anti-Proliferative Activities and Apoptosis Induction by Triterpenes Derived from Eriobotrya japonica in Human Leukemia Cell Lines
por: Uto, Takuhiro, et al.
Publicado: (2013)

Kayeassamin A Isolated from the Flower of Mammea siamensis Triggers Apoptosis by Activating Caspase-3/-8 in HL-60 Human Leukemia Cells
por: Uto, Takuhiro, et al.
Publicado: (2016)

Quassinoids from the Root of Eurycoma longifolia and Their Antiproliferative Activity on Human Cancer Cell Lines
por: Tung, Nguyen Huu, et al.
Publicado: (2017)

Liquiritin and Liquiritigenin Induce Melanogenesis via Enhancement of p38 and PKA Signaling Pathways
por: Uto, Takuhiro, et al.
Publicado: (2019)

Investigation of interfacial behavior of glycyrrhizin with a lipid raft model via a Langmuir monolayer study
por: Sakamoto, Seiichi, et al.
Publicado: (2013)

Steroidal Saponins Isolated from the Rhizome of Dioscorea tokoro Inhibit Cell Growth and Autophagy in Hepatocellular Carcinoma Cells
por: Okubo, Shinya, et al.
Publicado: (2021)

Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites
por: Sakamoto, Seiichi, et al.
Publicado: (2017)

Correction to: Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites
por: Sakamoto, Seiichi, et al.
Publicado: (2018)

Screening of Crude Drugs Used in Japanese Kampo Formulas for Autophagy-Mediated Cell Survival of the Human Hepatocellular Carcinoma Cell Line
por: Okubo, Shinya, et al.
Publicado: (2019)

Studies on Natural Products Using Monoclonal Antibodies: A Review
por: Shoyama, Yukihiro
Publicado: (2021)

In vitro fertilization using sperm activated by ML‐2–3 isolated from Morinda lucida Bentham leaves
por: Ohta, Tomoe, et al.
Publicado: (2022)

De-Glycyrrhizinated Licorice Extract Attenuates High Glucose-Stimulated Renal Tubular Epithelial–Mesenchymal Transition via Suppressing the Notch2 Signaling Pathway
por: Hsu, Yung-Chien, et al.
Publicado: (2020)

In vitro anti-Leishmania activity of tetracyclic iridoids from Morinda lucida, benth
por: Amoa-Bosompem, Michael, et al.
Publicado: (2016)

Dietary Crocin Inhibits Colitis and Colitis-Associated Colorectal Carcinogenesis in Male ICR Mice
por: Kawabata, Kunihiro, et al.
Publicado: (2012)

Antitrypanosomal Activities and Mechanisms of Action of Novel Tetracyclic Iridoids from Morinda lucida Benth.
por: Kwofie, Kofi D., et al.
Publicado: (2016)

Bioimprinting as a Receptor for Detection of Kwakhurin
por: Sakamoto, Seiichi, et al.
Publicado: (2022)

A New Highly Selective and Specific Anti-puerarin polyclonal Antibody for Determination of Puerarin Using a Mannich Reaction Hapten Conjugate
por: Udomsin, Orapin, et al.
Publicado: (2017)

Development of a colorless Centella asiatica (L.) Urb. extract using a natural deep eutectic solvent (NADES) and microwave-assisted extraction (MAE) optimized by response surface methodology
por: Phaisan, Suppalak, et al.
Publicado: (2021)

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review
por: Nuntawong, Poomraphie, et al.
Publicado: (2022)

BlotIt—Optimal alignment of Western blot and qPCR experiments
por: Kemmer, Svenja, et al.
Publicado: (2022)

Quantitative Detection of µ Opioid Receptor: Western Blot Analyses Using µ Opioid Receptor Knockout Mice
por: Kasai, Shinya, et al.
Publicado: (2011)

Preventing Dementia Using Saffron, The Kampo Medicine, Kamiuntanto, and Their Combination, Kamiuntantokabankoka
por: Kuchta, Kenny, et al.
Publicado: (2022)

The Blot upon the Brain
Publicado: (1885)

The Blot upon the Brain
Publicado: (1886)

Immunoblotting and dot blotting
por: Stott, D.I.
Publicado: (1989)

Development of EMab-51, a Sensitive and Specific Anti-Epidermal Growth Factor Receptor Monoclonal Antibody in Flow Cytometry, Western Blot, and Immunohistochemistry
por: Itai, Shunsuke, et al.
Publicado: (2017)

Improving rigor and reproducibility in western blot experiments with the blotRig analysis software
por: Omondi, Cleopa, et al.
Publicado: (2023)

Single-cell western blotting
por: Hughes, Alex J., et al.
Publicado: (2014)

Comprehensive Optimization of Western Blotting
por: Liu, Dishiwen, et al.
Publicado: (2023)

Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation
por: Inyai, Chadathorn, et al.
Publicado: (2020)

Transformation of Pueraria candollei var. mirifica phytoestrogens using immobilized and free β-glucosidase, a technique for enhancing estrogenic activity
por: Makkliang, Fonthip, et al.
Publicado: (2021)

Analysis of canthin-6-one alkaloids derived from Eurycoma spp. by micellar liquid chromatography and conventional high-performance liquid chromatography: a comparative evaluation
por: Sakdamas, Attapon, et al.
Publicado: (2023)

Correction: Analysis of canthin-6-one alkaloids derived from Eurycoma spp. by micellar liquid chromatography and conventional high-performance liquid chromatography: a comparative evaluation
por: Sakdamas, Attapon, et al.
Publicado: (2023)

Preparation of monoclonal antibody against human KIAA0100 protein and Northern blot analysis of human KIAA0100 gene
por: Cui, He, et al.
Publicado: (2017)

Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS)
por: Gilda, Jennifer E., et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_c7evq9mrmn2is9luhc3sp9kuks