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Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs
Autologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating the development of alternative therapeutics. Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together to induce ra...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296315/ https://www.ncbi.nlm.nih.gov/pubmed/22500171 http://dx.doi.org/10.1155/2012/861794 |
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author | Mumaw, Jennifer Jordan, Erin T. Sonnet, Corinne Olabisi, Ronke M. Olmsted-Davis, Elizabeth A. Davis, Alan R. Peroni, John F. West, Jennifer L. West, Franklin Lu, Yangqing Stice, Steven L. |
author_facet | Mumaw, Jennifer Jordan, Erin T. Sonnet, Corinne Olabisi, Ronke M. Olmsted-Davis, Elizabeth A. Davis, Alan R. Peroni, John F. West, Jennifer L. West, Franklin Lu, Yangqing Stice, Steven L. |
author_sort | Mumaw, Jennifer |
collection | PubMed |
description | Autologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating the development of alternative therapeutics. Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together to induce rapid bone formation. However, methods to make these treatments available for clinical applications are presently lacking. In this study we used mesenchymal stem cells (MSCs) due to their ease of harvest, replication potential, and immunomodulatory capabilities. MSCs were from sheep and pig due to their appeal as large animal models for bone nonunion. We demonstrated that cryopreservation of these microencapsulated MSCs did not affect their cell viability, adenoviral BMP2 production, or ability to initiate bone formation. Additionally, microspheres showed no appreciable damage from cryopreservation when examined with light and electron microscopy. These results validate the use of cryopreservation in preserving the viability and functionality of PEG-encapsulated BMP2-transduced MSCs. |
format | Online Article Text |
id | pubmed-3296315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-32963152012-04-12 Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs Mumaw, Jennifer Jordan, Erin T. Sonnet, Corinne Olabisi, Ronke M. Olmsted-Davis, Elizabeth A. Davis, Alan R. Peroni, John F. West, Jennifer L. West, Franklin Lu, Yangqing Stice, Steven L. Int J Biomater Research Article Autologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating the development of alternative therapeutics. Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together to induce rapid bone formation. However, methods to make these treatments available for clinical applications are presently lacking. In this study we used mesenchymal stem cells (MSCs) due to their ease of harvest, replication potential, and immunomodulatory capabilities. MSCs were from sheep and pig due to their appeal as large animal models for bone nonunion. We demonstrated that cryopreservation of these microencapsulated MSCs did not affect their cell viability, adenoviral BMP2 production, or ability to initiate bone formation. Additionally, microspheres showed no appreciable damage from cryopreservation when examined with light and electron microscopy. These results validate the use of cryopreservation in preserving the viability and functionality of PEG-encapsulated BMP2-transduced MSCs. Hindawi Publishing Corporation 2012 2012-02-07 /pmc/articles/PMC3296315/ /pubmed/22500171 http://dx.doi.org/10.1155/2012/861794 Text en Copyright © 2012 Jennifer Mumaw et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mumaw, Jennifer Jordan, Erin T. Sonnet, Corinne Olabisi, Ronke M. Olmsted-Davis, Elizabeth A. Davis, Alan R. Peroni, John F. West, Jennifer L. West, Franklin Lu, Yangqing Stice, Steven L. Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
title | Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
title_full | Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
title_fullStr | Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
title_full_unstemmed | Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
title_short | Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
title_sort | rapid heterotrophic ossification with cryopreserved poly(ethylene glycol-) microencapsulated bmp2-expressing mscs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296315/ https://www.ncbi.nlm.nih.gov/pubmed/22500171 http://dx.doi.org/10.1155/2012/861794 |
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