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Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs

BACKGROUND AIMS: Transplantation of synovial mesenchymal stromal cells (MSCs) may induce repair of cartilage defects. We transplanted synovial MSCs into cartilage defects using a simple method and investigated its usefulness and repair process in a pig model. METHODS: The chondrogenic potential of t...

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Autores principales: Nakamura, Tomomasa, Sekiya, Ichiro, Muneta, Takeshi, Hatsushika, Daisuke, Horie, Masafumi, Tsuji, Kunikazu, Kawarasaki, Tatsuo, Watanabe, Atsuya, Hishikawa, Shuji, Fujimoto, Yasuhiro, Tanaka, Hozumi, Kobayashi, Eiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Informa Healthcare 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296518/
https://www.ncbi.nlm.nih.gov/pubmed/22309371
http://dx.doi.org/10.3109/14653249.2011.638912
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author Nakamura, Tomomasa
Sekiya, Ichiro
Muneta, Takeshi
Hatsushika, Daisuke
Horie, Masafumi
Tsuji, Kunikazu
Kawarasaki, Tatsuo
Watanabe, Atsuya
Hishikawa, Shuji
Fujimoto, Yasuhiro
Tanaka, Hozumi
Kobayashi, Eiji
author_facet Nakamura, Tomomasa
Sekiya, Ichiro
Muneta, Takeshi
Hatsushika, Daisuke
Horie, Masafumi
Tsuji, Kunikazu
Kawarasaki, Tatsuo
Watanabe, Atsuya
Hishikawa, Shuji
Fujimoto, Yasuhiro
Tanaka, Hozumi
Kobayashi, Eiji
author_sort Nakamura, Tomomasa
collection PubMed
description BACKGROUND AIMS: Transplantation of synovial mesenchymal stromal cells (MSCs) may induce repair of cartilage defects. We transplanted synovial MSCs into cartilage defects using a simple method and investigated its usefulness and repair process in a pig model. METHODS: The chondrogenic potential of the porcine MSCs was compared in vitro. Cartilage defects were created in both knees of seven pigs, and divided into MSCs treated and non-treated control knees. Synovial MSCs were injected into the defect, and the knee was kept immobilized for 10 min before wound closure. To visualize the actual delivery and adhesion of the cells, fluorescence-labeled synovial MSCs from transgenic green fluorescent protein (GFP) pig were injected into the defect in a subgroup of two pigs. In these two animals, the wounds were closed before MSCs were injected and observed for 10 min under arthroscopic control. The defects were analyzed sequentially arthroscopically, histologically and by magnetic resonance imaging (MRI) for 3 months. RESULTS: Synovial MSCs had a higher chondrogenic potential in vitro than the other MSCs examined. Arthroscopic observations showed adhesion of synovial MSCs and membrane formation on the cartilage defects before cartilage repair. Quantification analyses for arthroscopy, histology and MRI revealed a better outcome in the MSC-treated knees than in the non-treated control knees. CONCLUSIONS: Leaving a synovial MSC suspension in cartilage defects for 10 min made it possible for cells to adhere in the defect in a porcine cartilage defect model. The cartilage defect was first covered with membrane, then the cartilage matrix emerged after transplantation of synovial MSCs.
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spelling pubmed-32965182012-03-22 Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs Nakamura, Tomomasa Sekiya, Ichiro Muneta, Takeshi Hatsushika, Daisuke Horie, Masafumi Tsuji, Kunikazu Kawarasaki, Tatsuo Watanabe, Atsuya Hishikawa, Shuji Fujimoto, Yasuhiro Tanaka, Hozumi Kobayashi, Eiji Cytotherapy Articles BACKGROUND AIMS: Transplantation of synovial mesenchymal stromal cells (MSCs) may induce repair of cartilage defects. We transplanted synovial MSCs into cartilage defects using a simple method and investigated its usefulness and repair process in a pig model. METHODS: The chondrogenic potential of the porcine MSCs was compared in vitro. Cartilage defects were created in both knees of seven pigs, and divided into MSCs treated and non-treated control knees. Synovial MSCs were injected into the defect, and the knee was kept immobilized for 10 min before wound closure. To visualize the actual delivery and adhesion of the cells, fluorescence-labeled synovial MSCs from transgenic green fluorescent protein (GFP) pig were injected into the defect in a subgroup of two pigs. In these two animals, the wounds were closed before MSCs were injected and observed for 10 min under arthroscopic control. The defects were analyzed sequentially arthroscopically, histologically and by magnetic resonance imaging (MRI) for 3 months. RESULTS: Synovial MSCs had a higher chondrogenic potential in vitro than the other MSCs examined. Arthroscopic observations showed adhesion of synovial MSCs and membrane formation on the cartilage defects before cartilage repair. Quantification analyses for arthroscopy, histology and MRI revealed a better outcome in the MSC-treated knees than in the non-treated control knees. CONCLUSIONS: Leaving a synovial MSC suspension in cartilage defects for 10 min made it possible for cells to adhere in the defect in a porcine cartilage defect model. The cartilage defect was first covered with membrane, then the cartilage matrix emerged after transplantation of synovial MSCs. Informa Healthcare 2012-03 2012-02-06 /pmc/articles/PMC3296518/ /pubmed/22309371 http://dx.doi.org/10.3109/14653249.2011.638912 Text en © 2012 Informa Healthcare http://creativecommons.org/licenses/by/2.0/ This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Informa Healthcare journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Nakamura, Tomomasa
Sekiya, Ichiro
Muneta, Takeshi
Hatsushika, Daisuke
Horie, Masafumi
Tsuji, Kunikazu
Kawarasaki, Tatsuo
Watanabe, Atsuya
Hishikawa, Shuji
Fujimoto, Yasuhiro
Tanaka, Hozumi
Kobayashi, Eiji
Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs
title Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs
title_full Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs
title_fullStr Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs
title_full_unstemmed Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs
title_short Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs
title_sort arthroscopic, histological and mri analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296518/
https://www.ncbi.nlm.nih.gov/pubmed/22309371
http://dx.doi.org/10.3109/14653249.2011.638912
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