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Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia
BACKGROUND: The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA). METHODS: Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two laboratories. A sub...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296606/ https://www.ncbi.nlm.nih.gov/pubmed/22316049 http://dx.doi.org/10.1186/1751-0147-54-10 |
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author | Piek, Christine J Teske, Erik van Leeuwen, Martin W Day, Michael J |
author_facet | Piek, Christine J Teske, Erik van Leeuwen, Martin W Day, Michael J |
author_sort | Piek, Christine J |
collection | PubMed |
description | BACKGROUND: The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA). METHODS: Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two laboratories. A subset of canine samples was categorized as having idiopathic IMHA, secondary IMHA, or no IMHA. RESULTS: The kappa values for agreement between the tests were in one laboratory 0.86 for canine and 0.58 for feline samples, and in the other 0.48 for canine samples. The lower agreement in the second laboratory was caused by a high number of positive canine DATs for which the gel test was negative. This group included significantly more dogs with secondary IMHA. CONCLUSIONS: The gel test might be used as a screening test for idiopathic IMHA and is less often positive in secondary IMHA than the DAT. |
format | Online Article Text |
id | pubmed-3296606 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32966062012-03-08 Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia Piek, Christine J Teske, Erik van Leeuwen, Martin W Day, Michael J Acta Vet Scand Research BACKGROUND: The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA). METHODS: Canine (n = 247) and feline (n = 74) blood samples were submitted for DAT testing to two laboratories. A subset of canine samples was categorized as having idiopathic IMHA, secondary IMHA, or no IMHA. RESULTS: The kappa values for agreement between the tests were in one laboratory 0.86 for canine and 0.58 for feline samples, and in the other 0.48 for canine samples. The lower agreement in the second laboratory was caused by a high number of positive canine DATs for which the gel test was negative. This group included significantly more dogs with secondary IMHA. CONCLUSIONS: The gel test might be used as a screening test for idiopathic IMHA and is less often positive in secondary IMHA than the DAT. BioMed Central 2012-02-08 /pmc/articles/PMC3296606/ /pubmed/22316049 http://dx.doi.org/10.1186/1751-0147-54-10 Text en Copyright ©2012 Piek et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Piek, Christine J Teske, Erik van Leeuwen, Martin W Day, Michael J Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia |
title | Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia |
title_full | Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia |
title_fullStr | Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia |
title_full_unstemmed | Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia |
title_short | Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia |
title_sort | good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296606/ https://www.ncbi.nlm.nih.gov/pubmed/22316049 http://dx.doi.org/10.1186/1751-0147-54-10 |
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