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Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells

BACKGROUND: The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported t...

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Autores principales: Mehrbod, Parvaneh, Ideris, Aini, Omar , Abdul Rahman, Hair-Bejo, Mohd, Tan, Sheau Wei, Kheiri, Masoumeh Tavassoti, Tabatabaian , Mansoureh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296628/
https://www.ncbi.nlm.nih.gov/pubmed/22340010
http://dx.doi.org/10.1186/1743-422X-9-44
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author Mehrbod, Parvaneh
Ideris, Aini
Omar , Abdul Rahman
Hair-Bejo, Mohd
Tan, Sheau Wei
Kheiri, Masoumeh Tavassoti
Tabatabaian , Mansoureh
author_facet Mehrbod, Parvaneh
Ideris, Aini
Omar , Abdul Rahman
Hair-Bejo, Mohd
Tan, Sheau Wei
Kheiri, Masoumeh Tavassoti
Tabatabaian , Mansoureh
author_sort Mehrbod, Parvaneh
collection PubMed
description BACKGROUND: The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported that the therapeutic properties of HESA-A are able to treat psoriasis vulgaris and cancers. However, no antiviral properties have been reported. METHODS: This study was designed to investigate the potential antiviral properties of HESA-A and its effects in modulating TNF-α and IL-6 cytokine levels. HESA-A was prepared in normal saline as a stock solution (0.8 mg/ml, pH = 7.4). Percentages of cell survival when exposed to different concentrations of HESA-A at different time intervals was determined by MTT assay. To study the potential antiviral activity of HESA-A, Madin-Darby Canine Kidney (MDCK) cells were treated with the effective concentration (EC(50)) of HESA-A (0.025 mg/ml) and 100 TCID(50)/0.1 ml of virus sample under different types of exposure. RESULTS: Based on the MTT method and hemagglutination assay (HA), HESA-A is capable of improving cell viability to 31% and decreasing HA titre to almost 99% in co-penetration exposures. In addition, based on quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), it was found that HESA-A causes decrements in TNF-α and IL-6 cytokine expressions, which was significant for TNF-α (p ≤ 0.05) but not for IL-6. CONCLUSION: In conclusion, HESA-A was effective against influenza infection through suppressing cytokine expression.
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spelling pubmed-32966282012-03-09 Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells Mehrbod, Parvaneh Ideris, Aini Omar , Abdul Rahman Hair-Bejo, Mohd Tan, Sheau Wei Kheiri, Masoumeh Tavassoti Tabatabaian , Mansoureh Virol J Research BACKGROUND: The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported that the therapeutic properties of HESA-A are able to treat psoriasis vulgaris and cancers. However, no antiviral properties have been reported. METHODS: This study was designed to investigate the potential antiviral properties of HESA-A and its effects in modulating TNF-α and IL-6 cytokine levels. HESA-A was prepared in normal saline as a stock solution (0.8 mg/ml, pH = 7.4). Percentages of cell survival when exposed to different concentrations of HESA-A at different time intervals was determined by MTT assay. To study the potential antiviral activity of HESA-A, Madin-Darby Canine Kidney (MDCK) cells were treated with the effective concentration (EC(50)) of HESA-A (0.025 mg/ml) and 100 TCID(50)/0.1 ml of virus sample under different types of exposure. RESULTS: Based on the MTT method and hemagglutination assay (HA), HESA-A is capable of improving cell viability to 31% and decreasing HA titre to almost 99% in co-penetration exposures. In addition, based on quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), it was found that HESA-A causes decrements in TNF-α and IL-6 cytokine expressions, which was significant for TNF-α (p ≤ 0.05) but not for IL-6. CONCLUSION: In conclusion, HESA-A was effective against influenza infection through suppressing cytokine expression. BioMed Central 2012-02-16 /pmc/articles/PMC3296628/ /pubmed/22340010 http://dx.doi.org/10.1186/1743-422X-9-44 Text en Copyright ©2012 Mehrbod et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Mehrbod, Parvaneh
Ideris, Aini
Omar , Abdul Rahman
Hair-Bejo, Mohd
Tan, Sheau Wei
Kheiri, Masoumeh Tavassoti
Tabatabaian , Mansoureh
Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells
title Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells
title_full Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells
title_fullStr Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells
title_full_unstemmed Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells
title_short Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells
title_sort attenuation of influenza virus infectivity with herbal-marine compound (hesa-a): an in vitro study in mdck cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296628/
https://www.ncbi.nlm.nih.gov/pubmed/22340010
http://dx.doi.org/10.1186/1743-422X-9-44
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