Cargando…
Parvovirus B19 DNA CpG Dinucleotide Methylation and Epigenetic Regulation of Viral Expression
CpG DNA methylation is one of the main epigenetic modifications playing a role in the control of gene expression. For DNA viruses whose genome has the ability to integrate in the host genome or to maintain as a latent episome, a correlation has been found between the extent of DNA methylation and vi...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296687/ https://www.ncbi.nlm.nih.gov/pubmed/22413013 http://dx.doi.org/10.1371/journal.pone.0033316 |
_version_ | 1782225775177498624 |
---|---|
author | Bonvicini, Francesca Manaresi, Elisabetta Di Furio, Francesca De Falco, Luisa Gallinella, Giorgio |
author_facet | Bonvicini, Francesca Manaresi, Elisabetta Di Furio, Francesca De Falco, Luisa Gallinella, Giorgio |
author_sort | Bonvicini, Francesca |
collection | PubMed |
description | CpG DNA methylation is one of the main epigenetic modifications playing a role in the control of gene expression. For DNA viruses whose genome has the ability to integrate in the host genome or to maintain as a latent episome, a correlation has been found between the extent of DNA methylation and viral quiescence. No information is available for Parvovirus B19, a human pathogenic virus, which is capable of both lytic and persistent infections. Within Parvovirus B19 genome, the inverted terminal regions display all the characteristic signatures of a genomic CpG island; therefore we hypothesised a role of CpG dinucleotide methylation in the regulation of viral genome expression. The analysis of CpG dinucleotide methylation of Parvovirus B19 DNA was carried out by an aptly designed quantitative real-time PCR assay on bisulfite-modified DNA. The effects of CpG methylation on the regulation of viral genome expression were first investigated by transfection of either unmethylated or in vitro methylated viral DNA in a model cell line, showing that methylation of viral DNA was correlated to lower expression levels of the viral genome. Then, in the course of in vitro infections in different cellular environments, it was observed that absence of viral expression and genome replication were both correlated to increasing levels of CpG methylation of viral DNA. Finally, the presence of CpG methylation was documented in viral DNA present in bioptic samples, indicating the occurrence and a possible role of this epigenetic modification in the course of natural infections. The presence of an epigenetic level of regulation of viral genome expression, possibly correlated to the silencing of the viral genome and contributing to the maintenance of the virus in tissues, can be relevant to the balance and outcome of the different types of infection associated to Parvovirus B19. |
format | Online Article Text |
id | pubmed-3296687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32966872012-03-12 Parvovirus B19 DNA CpG Dinucleotide Methylation and Epigenetic Regulation of Viral Expression Bonvicini, Francesca Manaresi, Elisabetta Di Furio, Francesca De Falco, Luisa Gallinella, Giorgio PLoS One Research Article CpG DNA methylation is one of the main epigenetic modifications playing a role in the control of gene expression. For DNA viruses whose genome has the ability to integrate in the host genome or to maintain as a latent episome, a correlation has been found between the extent of DNA methylation and viral quiescence. No information is available for Parvovirus B19, a human pathogenic virus, which is capable of both lytic and persistent infections. Within Parvovirus B19 genome, the inverted terminal regions display all the characteristic signatures of a genomic CpG island; therefore we hypothesised a role of CpG dinucleotide methylation in the regulation of viral genome expression. The analysis of CpG dinucleotide methylation of Parvovirus B19 DNA was carried out by an aptly designed quantitative real-time PCR assay on bisulfite-modified DNA. The effects of CpG methylation on the regulation of viral genome expression were first investigated by transfection of either unmethylated or in vitro methylated viral DNA in a model cell line, showing that methylation of viral DNA was correlated to lower expression levels of the viral genome. Then, in the course of in vitro infections in different cellular environments, it was observed that absence of viral expression and genome replication were both correlated to increasing levels of CpG methylation of viral DNA. Finally, the presence of CpG methylation was documented in viral DNA present in bioptic samples, indicating the occurrence and a possible role of this epigenetic modification in the course of natural infections. The presence of an epigenetic level of regulation of viral genome expression, possibly correlated to the silencing of the viral genome and contributing to the maintenance of the virus in tissues, can be relevant to the balance and outcome of the different types of infection associated to Parvovirus B19. Public Library of Science 2012-03-07 /pmc/articles/PMC3296687/ /pubmed/22413013 http://dx.doi.org/10.1371/journal.pone.0033316 Text en Bonvicini et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bonvicini, Francesca Manaresi, Elisabetta Di Furio, Francesca De Falco, Luisa Gallinella, Giorgio Parvovirus B19 DNA CpG Dinucleotide Methylation and Epigenetic Regulation of Viral Expression |
title | Parvovirus B19 DNA CpG Dinucleotide Methylation and Epigenetic Regulation of Viral Expression |
title_full | Parvovirus B19 DNA CpG Dinucleotide Methylation and Epigenetic Regulation of Viral Expression |
title_fullStr | Parvovirus B19 DNA CpG Dinucleotide Methylation and Epigenetic Regulation of Viral Expression |
title_full_unstemmed | Parvovirus B19 DNA CpG Dinucleotide Methylation and Epigenetic Regulation of Viral Expression |
title_short | Parvovirus B19 DNA CpG Dinucleotide Methylation and Epigenetic Regulation of Viral Expression |
title_sort | parvovirus b19 dna cpg dinucleotide methylation and epigenetic regulation of viral expression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296687/ https://www.ncbi.nlm.nih.gov/pubmed/22413013 http://dx.doi.org/10.1371/journal.pone.0033316 |
work_keys_str_mv | AT bonvicinifrancesca parvovirusb19dnacpgdinucleotidemethylationandepigeneticregulationofviralexpression AT manaresielisabetta parvovirusb19dnacpgdinucleotidemethylationandepigeneticregulationofviralexpression AT difuriofrancesca parvovirusb19dnacpgdinucleotidemethylationandepigeneticregulationofviralexpression AT defalcoluisa parvovirusb19dnacpgdinucleotidemethylationandepigeneticregulationofviralexpression AT gallinellagiorgio parvovirusb19dnacpgdinucleotidemethylationandepigeneticregulationofviralexpression |