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Different Infective Forms Trigger Distinct Immune Response in Experimental Chagas Disease

Although metacyclic and blood trypomastigotes are completely functional in relation to parasite-host interaction and/or target cell invasion, they differ in the molecules present on the surface. Thus, aspects related to the variability that the forms of T. cruzi interacts with host cells may lead to...

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Autores principales: Vieira, Paula Melo de Abreu, Francisco, Amanda Fortes, Machado, Evandro Marques de Meneses, Nogueira, Nívia Carolina, Fonseca, Kátia da Silva, Reis, Alexandre Barbosa, Teixeira-Carvalho, Andrea, Martins-Filho, Olindo Assis, Tafuri, Washington Luiz, Carneiro, Cláudia Martins
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296760/
https://www.ncbi.nlm.nih.gov/pubmed/22412949
http://dx.doi.org/10.1371/journal.pone.0032912
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author Vieira, Paula Melo de Abreu
Francisco, Amanda Fortes
Machado, Evandro Marques de Meneses
Nogueira, Nívia Carolina
Fonseca, Kátia da Silva
Reis, Alexandre Barbosa
Teixeira-Carvalho, Andrea
Martins-Filho, Olindo Assis
Tafuri, Washington Luiz
Carneiro, Cláudia Martins
author_facet Vieira, Paula Melo de Abreu
Francisco, Amanda Fortes
Machado, Evandro Marques de Meneses
Nogueira, Nívia Carolina
Fonseca, Kátia da Silva
Reis, Alexandre Barbosa
Teixeira-Carvalho, Andrea
Martins-Filho, Olindo Assis
Tafuri, Washington Luiz
Carneiro, Cláudia Martins
author_sort Vieira, Paula Melo de Abreu
collection PubMed
description Although metacyclic and blood trypomastigotes are completely functional in relation to parasite-host interaction and/or target cell invasion, they differ in the molecules present on the surface. Thus, aspects related to the variability that the forms of T. cruzi interacts with host cells may lead to fundamental implications on the immune response against this parasite and, consequently, the clinical evolution of Chagas disease. We have shown that BT infected mice presented higher levels of parasitemia during all the acute phase of infection. Moreover, the infection with either MT or BT forms resulted in increased levels of total leukocytes, monocytes and lymphocytes, specifically later for MT and earlier for BT. The infection with BT forms presented earlier production of proinflammatory cytokine TNF-α and later of IFN-γ by both T cells subpopulations. This event was accompanied by an early cardiac inflammation with an exacerbation of this process at the end of the acute phase. On the other hand, infection with MT forms result in an early production of IFN-γ, with subsequent control in the production of this cytokine by IL-10, which provided to these animals an immunomodulatory profile in the end of the acute phase. These results are in agreement with what was found for cardiac inflammation where animals infected with MT forms showed intense cardiac inflammation later at infection, with a decrease in the same at the end of this phase. In summary, our findings emphasize the importance of taking into account the inoculums source of T. cruzi, since vectorial or transfusional routes of T. cruzi infection may trigger distinct parasite-host interactions during the acute phase that may influence relevant biological aspects of chronic Chagas disease.
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spelling pubmed-32967602012-03-12 Different Infective Forms Trigger Distinct Immune Response in Experimental Chagas Disease Vieira, Paula Melo de Abreu Francisco, Amanda Fortes Machado, Evandro Marques de Meneses Nogueira, Nívia Carolina Fonseca, Kátia da Silva Reis, Alexandre Barbosa Teixeira-Carvalho, Andrea Martins-Filho, Olindo Assis Tafuri, Washington Luiz Carneiro, Cláudia Martins PLoS One Research Article Although metacyclic and blood trypomastigotes are completely functional in relation to parasite-host interaction and/or target cell invasion, they differ in the molecules present on the surface. Thus, aspects related to the variability that the forms of T. cruzi interacts with host cells may lead to fundamental implications on the immune response against this parasite and, consequently, the clinical evolution of Chagas disease. We have shown that BT infected mice presented higher levels of parasitemia during all the acute phase of infection. Moreover, the infection with either MT or BT forms resulted in increased levels of total leukocytes, monocytes and lymphocytes, specifically later for MT and earlier for BT. The infection with BT forms presented earlier production of proinflammatory cytokine TNF-α and later of IFN-γ by both T cells subpopulations. This event was accompanied by an early cardiac inflammation with an exacerbation of this process at the end of the acute phase. On the other hand, infection with MT forms result in an early production of IFN-γ, with subsequent control in the production of this cytokine by IL-10, which provided to these animals an immunomodulatory profile in the end of the acute phase. These results are in agreement with what was found for cardiac inflammation where animals infected with MT forms showed intense cardiac inflammation later at infection, with a decrease in the same at the end of this phase. In summary, our findings emphasize the importance of taking into account the inoculums source of T. cruzi, since vectorial or transfusional routes of T. cruzi infection may trigger distinct parasite-host interactions during the acute phase that may influence relevant biological aspects of chronic Chagas disease. Public Library of Science 2012-03-07 /pmc/articles/PMC3296760/ /pubmed/22412949 http://dx.doi.org/10.1371/journal.pone.0032912 Text en Vieira et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Vieira, Paula Melo de Abreu
Francisco, Amanda Fortes
Machado, Evandro Marques de Meneses
Nogueira, Nívia Carolina
Fonseca, Kátia da Silva
Reis, Alexandre Barbosa
Teixeira-Carvalho, Andrea
Martins-Filho, Olindo Assis
Tafuri, Washington Luiz
Carneiro, Cláudia Martins
Different Infective Forms Trigger Distinct Immune Response in Experimental Chagas Disease
title Different Infective Forms Trigger Distinct Immune Response in Experimental Chagas Disease
title_full Different Infective Forms Trigger Distinct Immune Response in Experimental Chagas Disease
title_fullStr Different Infective Forms Trigger Distinct Immune Response in Experimental Chagas Disease
title_full_unstemmed Different Infective Forms Trigger Distinct Immune Response in Experimental Chagas Disease
title_short Different Infective Forms Trigger Distinct Immune Response in Experimental Chagas Disease
title_sort different infective forms trigger distinct immune response in experimental chagas disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296760/
https://www.ncbi.nlm.nih.gov/pubmed/22412949
http://dx.doi.org/10.1371/journal.pone.0032912
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