Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms

Cholinesterase activity in blood of laboratory rats was monitored. Rats were intoxicated with paraoxon at dosis of 0 – 65 – 125 – 170 – 250 – 500 nmol. The 250 nmol dose was found to be the LD(50). An electrochemical sensor was found useful to provide information about cholinesterase activity. The d...

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Autores principales: Pohanka, Miroslav, Novotný, Ladislav, Misík, Jan, Kuca, Kamil, Zdarova-Karasova, Jana, Hrabinova, Martina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297120/
https://www.ncbi.nlm.nih.gov/pubmed/22412329
http://dx.doi.org/10.3390/s90503627
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author Pohanka, Miroslav
Novotný, Ladislav
Misík, Jan
Kuca, Kamil
Zdarova-Karasova, Jana
Hrabinova, Martina
author_facet Pohanka, Miroslav
Novotný, Ladislav
Misík, Jan
Kuca, Kamil
Zdarova-Karasova, Jana
Hrabinova, Martina
author_sort Pohanka, Miroslav
collection PubMed
description Cholinesterase activity in blood of laboratory rats was monitored. Rats were intoxicated with paraoxon at dosis of 0 – 65 – 125 – 170 – 250 – 500 nmol. The 250 nmol dose was found to be the LD(50). An electrochemical sensor was found useful to provide information about cholinesterase activity. The decrease of cholinesterase activity was correlated to intoxication symptoms and mortality level. It was found that the symptoms of intoxication are not observed while at least 50% of cholinesterase activity in blood remains. The minimal cholinesterase activity essential to survival is around 10%, when compared with the initial state. No changes in levels of low moleculary weight antioxidants were observed.
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spelling pubmed-32971202012-03-12 Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms Pohanka, Miroslav Novotný, Ladislav Misík, Jan Kuca, Kamil Zdarova-Karasova, Jana Hrabinova, Martina Sensors (Basel) Article Cholinesterase activity in blood of laboratory rats was monitored. Rats were intoxicated with paraoxon at dosis of 0 – 65 – 125 – 170 – 250 – 500 nmol. The 250 nmol dose was found to be the LD(50). An electrochemical sensor was found useful to provide information about cholinesterase activity. The decrease of cholinesterase activity was correlated to intoxication symptoms and mortality level. It was found that the symptoms of intoxication are not observed while at least 50% of cholinesterase activity in blood remains. The minimal cholinesterase activity essential to survival is around 10%, when compared with the initial state. No changes in levels of low moleculary weight antioxidants were observed. Molecular Diversity Preservation International (MDPI) 2009-05-14 /pmc/articles/PMC3297120/ /pubmed/22412329 http://dx.doi.org/10.3390/s90503627 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Pohanka, Miroslav
Novotný, Ladislav
Misík, Jan
Kuca, Kamil
Zdarova-Karasova, Jana
Hrabinova, Martina
Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms
title Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms
title_full Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms
title_fullStr Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms
title_full_unstemmed Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms
title_short Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms
title_sort evaluation of cholinesterase activities during in vivo intoxication using an electrochemical sensor strip – correlation with intoxication symptoms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297120/
https://www.ncbi.nlm.nih.gov/pubmed/22412329
http://dx.doi.org/10.3390/s90503627
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