Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems

The advent of nucleic acid-based pathogen detection methods offers increased sensitivity and specificity over traditional microbiological techniques, driving the development of portable, integrated biosensors. The miniaturization and automation of integrated detection systems presents a significant...

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Detalles Bibliográficos
Autores principales: Lui, Clarissa, Cady, Nathaniel C., Batt, Carl A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297159/
https://www.ncbi.nlm.nih.gov/pubmed/22412335
http://dx.doi.org/10.3390/s90503713
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author Lui, Clarissa
Cady, Nathaniel C.
Batt, Carl A.
author_facet Lui, Clarissa
Cady, Nathaniel C.
Batt, Carl A.
author_sort Lui, Clarissa
collection PubMed
description The advent of nucleic acid-based pathogen detection methods offers increased sensitivity and specificity over traditional microbiological techniques, driving the development of portable, integrated biosensors. The miniaturization and automation of integrated detection systems presents a significant advantage for rapid, portable field-based testing. In this review, we highlight current developments and directions in nucleic acid-based micro total analysis systems for the detection of bacterial pathogens. Recent progress in the miniaturization of microfluidic processing steps for cell capture, DNA extraction and purification, polymerase chain reaction, and product detection are detailed. Discussions include strategies and challenges for implementation of an integrated portable platform.
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spelling pubmed-32971592012-03-12 Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems Lui, Clarissa Cady, Nathaniel C. Batt, Carl A. Sensors (Basel) Review The advent of nucleic acid-based pathogen detection methods offers increased sensitivity and specificity over traditional microbiological techniques, driving the development of portable, integrated biosensors. The miniaturization and automation of integrated detection systems presents a significant advantage for rapid, portable field-based testing. In this review, we highlight current developments and directions in nucleic acid-based micro total analysis systems for the detection of bacterial pathogens. Recent progress in the miniaturization of microfluidic processing steps for cell capture, DNA extraction and purification, polymerase chain reaction, and product detection are detailed. Discussions include strategies and challenges for implementation of an integrated portable platform. Molecular Diversity Preservation International (MDPI) 2009-05-18 /pmc/articles/PMC3297159/ /pubmed/22412335 http://dx.doi.org/10.3390/s90503713 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Lui, Clarissa
Cady, Nathaniel C.
Batt, Carl A.
Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems
title Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems
title_full Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems
title_fullStr Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems
title_full_unstemmed Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems
title_short Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems
title_sort nucleic acid-based detection of bacterial pathogens using integrated microfluidic platform systems
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297159/
https://www.ncbi.nlm.nih.gov/pubmed/22412335
http://dx.doi.org/10.3390/s90503713
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AT cadynathanielc nucleicacidbaseddetectionofbacterialpathogensusingintegratedmicrofluidicplatformsystems
AT battcarla nucleicacidbaseddetectionofbacterialpathogensusingintegratedmicrofluidicplatformsystems

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