Cargando…

Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging

Here we describe an automated method, which we call serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and ca...

Descripción completa

Detalles Bibliográficos
Autores principales: Ragan, Timothy, Kadiri, Lolahon R., Venkataraju, Kannan Umadevi, Bahlmann, Karsten, Sutin, Jason, Taranda, Julian, Arganda-Carreras, Ignacio, Kim, Yongsoo, Seung, H. Sebastian, Osten, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297424/
https://www.ncbi.nlm.nih.gov/pubmed/22245809
http://dx.doi.org/10.1038/nmeth.1854
Descripción
Sumario:Here we describe an automated method, which we call serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in 3D, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.