Cargando…
Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging
Here we describe an automated method, which we call serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and ca...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2012
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297424/ https://www.ncbi.nlm.nih.gov/pubmed/22245809 http://dx.doi.org/10.1038/nmeth.1854 |
| _version_ | 1782225870887321600 |
|---|---|
| author | Ragan, Timothy Kadiri, Lolahon R. Venkataraju, Kannan Umadevi Bahlmann, Karsten Sutin, Jason Taranda, Julian Arganda-Carreras, Ignacio Kim, Yongsoo Seung, H. Sebastian Osten, Pavel |
| author_facet | Ragan, Timothy Kadiri, Lolahon R. Venkataraju, Kannan Umadevi Bahlmann, Karsten Sutin, Jason Taranda, Julian Arganda-Carreras, Ignacio Kim, Yongsoo Seung, H. Sebastian Osten, Pavel |
| author_sort | Ragan, Timothy |
| collection | PubMed |
| description | Here we describe an automated method, which we call serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in 3D, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders. |
| format | Online Article Text |
| id | pubmed-3297424 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2012 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-32974242012-09-01 Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging Ragan, Timothy Kadiri, Lolahon R. Venkataraju, Kannan Umadevi Bahlmann, Karsten Sutin, Jason Taranda, Julian Arganda-Carreras, Ignacio Kim, Yongsoo Seung, H. Sebastian Osten, Pavel Nat Methods Article Here we describe an automated method, which we call serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in 3D, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders. 2012-01-15 /pmc/articles/PMC3297424/ /pubmed/22245809 http://dx.doi.org/10.1038/nmeth.1854 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Ragan, Timothy Kadiri, Lolahon R. Venkataraju, Kannan Umadevi Bahlmann, Karsten Sutin, Jason Taranda, Julian Arganda-Carreras, Ignacio Kim, Yongsoo Seung, H. Sebastian Osten, Pavel Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging |
| title | Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging |
| title_full | Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging |
| title_fullStr | Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging |
| title_full_unstemmed | Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging |
| title_short | Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging |
| title_sort | serial two-photon tomography: an automated method for ex-vivo mouse brain imaging |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297424/ https://www.ncbi.nlm.nih.gov/pubmed/22245809 http://dx.doi.org/10.1038/nmeth.1854 |
| work_keys_str_mv | AT ragantimothy serialtwophotontomographyanautomatedmethodforexvivomousebrainimaging AT kadirilolahonr serialtwophotontomographyanautomatedmethodforexvivomousebrainimaging AT venkatarajukannanumadevi serialtwophotontomographyanautomatedmethodforexvivomousebrainimaging AT bahlmannkarsten serialtwophotontomographyanautomatedmethodforexvivomousebrainimaging AT sutinjason serialtwophotontomographyanautomatedmethodforexvivomousebrainimaging AT tarandajulian serialtwophotontomographyanautomatedmethodforexvivomousebrainimaging AT argandacarrerasignacio serialtwophotontomographyanautomatedmethodforexvivomousebrainimaging AT kimyongsoo serialtwophotontomographyanautomatedmethodforexvivomousebrainimaging AT seunghsebastian serialtwophotontomographyanautomatedmethodforexvivomousebrainimaging AT ostenpavel serialtwophotontomographyanautomatedmethodforexvivomousebrainimaging |