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A novel, simple, and sensitive colorimetric method to determine aromatic amino acid aminotransferase activity using the Salkowski reagent

This study describes the development of a new colorimetric assay to determine aromatic amino acid aminotransferase (ArAT) activity. The assay is based on the transamination of l-tryptophan in the presence of 2-oxoglutarate, which yields indole-3-pyruvate (IPyA). The amount of IPyA formed was quantif...

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Detalles Bibliográficos
Autores principales:	Szkop, Michał, Sikora, Piotr, Orzechowski, Sławomir
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Netherlands 2011
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297745/ https://www.ncbi.nlm.nih.gov/pubmed/22130693 http://dx.doi.org/10.1007/s12223-011-0089-y

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