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Rapid Identification of Emerging Pathogens: Coronavirus

We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrome...

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Autores principales: Sampath, Rangarajan, Hofstadler, Steven A., Blyn, Lawrence B., Eshoo, Mark W., Hall, Thomas A., Massire, Christian, Levene, Harold M., Hannis, James C., Harrell, Patina M., Neuman, Benjamin, Buchmeier, Michael J., Jiang, Yun, Ranken, Raymond, Drader, Jared J., Samant, Vivek, Griffey, Richard H., McNeil, John A., Crooke, Stanley T., Ecker, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298233/
https://www.ncbi.nlm.nih.gov/pubmed/15757550
http://dx.doi.org/10.3201/eid1103.040629
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author Sampath, Rangarajan
Hofstadler, Steven A.
Blyn, Lawrence B.
Eshoo, Mark W.
Hall, Thomas A.
Massire, Christian
Levene, Harold M.
Hannis, James C.
Harrell, Patina M.
Neuman, Benjamin
Buchmeier, Michael J.
Jiang, Yun
Ranken, Raymond
Drader, Jared J.
Samant, Vivek
Griffey, Richard H.
McNeil, John A.
Crooke, Stanley T.
Ecker, David J.
author_facet Sampath, Rangarajan
Hofstadler, Steven A.
Blyn, Lawrence B.
Eshoo, Mark W.
Hall, Thomas A.
Massire, Christian
Levene, Harold M.
Hannis, James C.
Harrell, Patina M.
Neuman, Benjamin
Buchmeier, Michael J.
Jiang, Yun
Ranken, Raymond
Drader, Jared J.
Samant, Vivek
Griffey, Richard H.
McNeil, John A.
Crooke, Stanley T.
Ecker, David J.
author_sort Sampath, Rangarajan
collection PubMed
description We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was ≈1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day.
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spelling pubmed-32982332012-03-12 Rapid Identification of Emerging Pathogens: Coronavirus Sampath, Rangarajan Hofstadler, Steven A. Blyn, Lawrence B. Eshoo, Mark W. Hall, Thomas A. Massire, Christian Levene, Harold M. Hannis, James C. Harrell, Patina M. Neuman, Benjamin Buchmeier, Michael J. Jiang, Yun Ranken, Raymond Drader, Jared J. Samant, Vivek Griffey, Richard H. McNeil, John A. Crooke, Stanley T. Ecker, David J. Emerg Infect Dis Research We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was ≈1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day. Centers for Disease Control and Prevention 2005-03 /pmc/articles/PMC3298233/ /pubmed/15757550 http://dx.doi.org/10.3201/eid1103.040629 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Sampath, Rangarajan
Hofstadler, Steven A.
Blyn, Lawrence B.
Eshoo, Mark W.
Hall, Thomas A.
Massire, Christian
Levene, Harold M.
Hannis, James C.
Harrell, Patina M.
Neuman, Benjamin
Buchmeier, Michael J.
Jiang, Yun
Ranken, Raymond
Drader, Jared J.
Samant, Vivek
Griffey, Richard H.
McNeil, John A.
Crooke, Stanley T.
Ecker, David J.
Rapid Identification of Emerging Pathogens: Coronavirus
title Rapid Identification of Emerging Pathogens: Coronavirus
title_full Rapid Identification of Emerging Pathogens: Coronavirus
title_fullStr Rapid Identification of Emerging Pathogens: Coronavirus
title_full_unstemmed Rapid Identification of Emerging Pathogens: Coronavirus
title_short Rapid Identification of Emerging Pathogens: Coronavirus
title_sort rapid identification of emerging pathogens: coronavirus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298233/
https://www.ncbi.nlm.nih.gov/pubmed/15757550
http://dx.doi.org/10.3201/eid1103.040629
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