Cargando…
Rapid Identification of Emerging Pathogens: Coronavirus
We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrome...
Autores principales: | , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Centers for Disease Control and Prevention
2005
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298233/ https://www.ncbi.nlm.nih.gov/pubmed/15757550 http://dx.doi.org/10.3201/eid1103.040629 |
_version_ | 1782225962873651200 |
---|---|
author | Sampath, Rangarajan Hofstadler, Steven A. Blyn, Lawrence B. Eshoo, Mark W. Hall, Thomas A. Massire, Christian Levene, Harold M. Hannis, James C. Harrell, Patina M. Neuman, Benjamin Buchmeier, Michael J. Jiang, Yun Ranken, Raymond Drader, Jared J. Samant, Vivek Griffey, Richard H. McNeil, John A. Crooke, Stanley T. Ecker, David J. |
author_facet | Sampath, Rangarajan Hofstadler, Steven A. Blyn, Lawrence B. Eshoo, Mark W. Hall, Thomas A. Massire, Christian Levene, Harold M. Hannis, James C. Harrell, Patina M. Neuman, Benjamin Buchmeier, Michael J. Jiang, Yun Ranken, Raymond Drader, Jared J. Samant, Vivek Griffey, Richard H. McNeil, John A. Crooke, Stanley T. Ecker, David J. |
author_sort | Sampath, Rangarajan |
collection | PubMed |
description | We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was ≈1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day. |
format | Online Article Text |
id | pubmed-3298233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-32982332012-03-12 Rapid Identification of Emerging Pathogens: Coronavirus Sampath, Rangarajan Hofstadler, Steven A. Blyn, Lawrence B. Eshoo, Mark W. Hall, Thomas A. Massire, Christian Levene, Harold M. Hannis, James C. Harrell, Patina M. Neuman, Benjamin Buchmeier, Michael J. Jiang, Yun Ranken, Raymond Drader, Jared J. Samant, Vivek Griffey, Richard H. McNeil, John A. Crooke, Stanley T. Ecker, David J. Emerg Infect Dis Research We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was ≈1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day. Centers for Disease Control and Prevention 2005-03 /pmc/articles/PMC3298233/ /pubmed/15757550 http://dx.doi.org/10.3201/eid1103.040629 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Sampath, Rangarajan Hofstadler, Steven A. Blyn, Lawrence B. Eshoo, Mark W. Hall, Thomas A. Massire, Christian Levene, Harold M. Hannis, James C. Harrell, Patina M. Neuman, Benjamin Buchmeier, Michael J. Jiang, Yun Ranken, Raymond Drader, Jared J. Samant, Vivek Griffey, Richard H. McNeil, John A. Crooke, Stanley T. Ecker, David J. Rapid Identification of Emerging Pathogens: Coronavirus |
title | Rapid Identification of Emerging Pathogens: Coronavirus |
title_full | Rapid Identification of Emerging Pathogens: Coronavirus |
title_fullStr | Rapid Identification of Emerging Pathogens: Coronavirus |
title_full_unstemmed | Rapid Identification of Emerging Pathogens: Coronavirus |
title_short | Rapid Identification of Emerging Pathogens: Coronavirus |
title_sort | rapid identification of emerging pathogens: coronavirus |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298233/ https://www.ncbi.nlm.nih.gov/pubmed/15757550 http://dx.doi.org/10.3201/eid1103.040629 |
work_keys_str_mv | AT sampathrangarajan rapididentificationofemergingpathogenscoronavirus AT hofstadlerstevena rapididentificationofemergingpathogenscoronavirus AT blynlawrenceb rapididentificationofemergingpathogenscoronavirus AT eshoomarkw rapididentificationofemergingpathogenscoronavirus AT hallthomasa rapididentificationofemergingpathogenscoronavirus AT massirechristian rapididentificationofemergingpathogenscoronavirus AT leveneharoldm rapididentificationofemergingpathogenscoronavirus AT hannisjamesc rapididentificationofemergingpathogenscoronavirus AT harrellpatinam rapididentificationofemergingpathogenscoronavirus AT neumanbenjamin rapididentificationofemergingpathogenscoronavirus AT buchmeiermichaelj rapididentificationofemergingpathogenscoronavirus AT jiangyun rapididentificationofemergingpathogenscoronavirus AT rankenraymond rapididentificationofemergingpathogenscoronavirus AT draderjaredj rapididentificationofemergingpathogenscoronavirus AT samantvivek rapididentificationofemergingpathogenscoronavirus AT griffeyrichardh rapididentificationofemergingpathogenscoronavirus AT mcneiljohna rapididentificationofemergingpathogenscoronavirus AT crookestanleyt rapididentificationofemergingpathogenscoronavirus AT eckerdavidj rapididentificationofemergingpathogenscoronavirus |