Identifying Relapsing Fever Borrelia, Senegal

We describe a nested polymerase chain reaction for the identification of Borrelia species from serum of patients with unidentified fevers. This technique, based on single nucleotide polymorphisms of the 16S ribosomal RNA gene, was used to test blood samples from 7,750 patients, 33 of whom were diagn...

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Detalles Bibliográficos
Autores principales: Brahim, Hamoud, Perrier-Gros-Claude, Jean David, Postic, Danielle, Baranton, Guy, Jambou, Ronan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2005
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298260/
https://www.ncbi.nlm.nih.gov/pubmed/15757570
http://dx.doi.org/10.3201/eid1103.040506
Descripción
Sumario:We describe a nested polymerase chain reaction for the identification of Borrelia species from serum of patients with unidentified fevers. This technique, based on single nucleotide polymorphisms of the 16S ribosomal RNA gene, was used to test blood samples from 7,750 patients, 33 of whom were diagnosed with spirochete infections. Borrelia crocidurae was the only species identified.

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