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Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis
BACKGROUND: Essential Thrombocythemia (ET) and Primary Myelofibrosis (PMF) are Chronic Myeloproliferative Neoplasms (MPN) characterized by clonal myeloproliferation/myeloaccumulation without cell maturation impairment. The JAK2 V617F mutation and PRV1 gene overexpression may contribute to MPN physio...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298534/ https://www.ncbi.nlm.nih.gov/pubmed/22300941 http://dx.doi.org/10.1186/1756-8722-5-2 |
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author | Tognon, Raquel Gasparotto, Elainy PL Neves, Renata P Nunes, Natália S Ferreira, Aline F Palma, Patrícia VB Kashima, Simone Covas, Dimas T Santana, Mary Souto, Elizabeth X Zanichelli, Maria Aparecida Simões, Belinda P de Souza, Ana Maria Castro, Fabíola A |
author_facet | Tognon, Raquel Gasparotto, Elainy PL Neves, Renata P Nunes, Natália S Ferreira, Aline F Palma, Patrícia VB Kashima, Simone Covas, Dimas T Santana, Mary Souto, Elizabeth X Zanichelli, Maria Aparecida Simões, Belinda P de Souza, Ana Maria Castro, Fabíola A |
author_sort | Tognon, Raquel |
collection | PubMed |
description | BACKGROUND: Essential Thrombocythemia (ET) and Primary Myelofibrosis (PMF) are Chronic Myeloproliferative Neoplasms (MPN) characterized by clonal myeloproliferation/myeloaccumulation without cell maturation impairment. The JAK2 V617F mutation and PRV1 gene overexpression may contribute to MPN physiopathology. We hypothesized that deregulation of the apoptotic machinery may also play a role in the pathogenesis of ET and PMF. In this study we evaluated the apoptosis-related gene and protein expression of BCL2 family members in bone marrow CD34(+ )hematopoietic stem cells (HSC) and peripheral blood leukocytes from ET and PMF patients. We also tested whether the gene expression results were correlated with JAK2 V617F allele burden percentage, PRV1 overexpression, and clinical and laboratory parameters. RESULTS: By real time PCR assay, we observed that A1, MCL1, BIK and BID, as well as A1, BCLW and BAK gene expression were increased in ET and PMF CD34(+ )cells respectively, while pro-apoptotic BAX and anti-apoptotic BCL2 mRNA levels were found to be lower in ET and PMF CD34(+ )cells respectively, in relation to controls. In patients' leukocytes, we detected an upregulation of anti-apoptotic genes A1, BCL2, BCL-X(L )and BCLW. In contrast, pro-apoptotic BID and BIM(EL )expression were downregulated in ET leukocytes. Increased BCL-X(L )protein expression in PMF leukocytes and decreased BID protein expression in ET leukocytes were observed by Western Blot. In ET leukocytes, we found a correlation between JAK2 V617F allele burden and BAX, BIK and BAD gene expression and between A1, BAX and BIK and PRV1 gene expression. A negative correlation between PRV1 gene expression and platelet count was observed, as well as a positive correlation between PRV1 gene expression and splenomegaly. CONCLUSIONS: Our results suggest the participation of intrinsic apoptosis pathway in the MPN physiopathology. In addition, PRV1 and JAK2 V617F allele burden were linked to deregulation of the apoptotic machinery. |
format | Online Article Text |
id | pubmed-3298534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32985342012-03-10 Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis Tognon, Raquel Gasparotto, Elainy PL Neves, Renata P Nunes, Natália S Ferreira, Aline F Palma, Patrícia VB Kashima, Simone Covas, Dimas T Santana, Mary Souto, Elizabeth X Zanichelli, Maria Aparecida Simões, Belinda P de Souza, Ana Maria Castro, Fabíola A J Hematol Oncol Research BACKGROUND: Essential Thrombocythemia (ET) and Primary Myelofibrosis (PMF) are Chronic Myeloproliferative Neoplasms (MPN) characterized by clonal myeloproliferation/myeloaccumulation without cell maturation impairment. The JAK2 V617F mutation and PRV1 gene overexpression may contribute to MPN physiopathology. We hypothesized that deregulation of the apoptotic machinery may also play a role in the pathogenesis of ET and PMF. In this study we evaluated the apoptosis-related gene and protein expression of BCL2 family members in bone marrow CD34(+ )hematopoietic stem cells (HSC) and peripheral blood leukocytes from ET and PMF patients. We also tested whether the gene expression results were correlated with JAK2 V617F allele burden percentage, PRV1 overexpression, and clinical and laboratory parameters. RESULTS: By real time PCR assay, we observed that A1, MCL1, BIK and BID, as well as A1, BCLW and BAK gene expression were increased in ET and PMF CD34(+ )cells respectively, while pro-apoptotic BAX and anti-apoptotic BCL2 mRNA levels were found to be lower in ET and PMF CD34(+ )cells respectively, in relation to controls. In patients' leukocytes, we detected an upregulation of anti-apoptotic genes A1, BCL2, BCL-X(L )and BCLW. In contrast, pro-apoptotic BID and BIM(EL )expression were downregulated in ET leukocytes. Increased BCL-X(L )protein expression in PMF leukocytes and decreased BID protein expression in ET leukocytes were observed by Western Blot. In ET leukocytes, we found a correlation between JAK2 V617F allele burden and BAX, BIK and BAD gene expression and between A1, BAX and BIK and PRV1 gene expression. A negative correlation between PRV1 gene expression and platelet count was observed, as well as a positive correlation between PRV1 gene expression and splenomegaly. CONCLUSIONS: Our results suggest the participation of intrinsic apoptosis pathway in the MPN physiopathology. In addition, PRV1 and JAK2 V617F allele burden were linked to deregulation of the apoptotic machinery. BioMed Central 2012-02-02 /pmc/articles/PMC3298534/ /pubmed/22300941 http://dx.doi.org/10.1186/1756-8722-5-2 Text en Copyright ©2012 Tognon et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Tognon, Raquel Gasparotto, Elainy PL Neves, Renata P Nunes, Natália S Ferreira, Aline F Palma, Patrícia VB Kashima, Simone Covas, Dimas T Santana, Mary Souto, Elizabeth X Zanichelli, Maria Aparecida Simões, Belinda P de Souza, Ana Maria Castro, Fabíola A Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis |
title | Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis |
title_full | Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis |
title_fullStr | Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis |
title_full_unstemmed | Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis |
title_short | Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis |
title_sort | deregulation of apoptosis-related genes is associated with prv1 overexpression and jak2 v617f allele burden in essential thrombocythemia and myelofibrosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298534/ https://www.ncbi.nlm.nih.gov/pubmed/22300941 http://dx.doi.org/10.1186/1756-8722-5-2 |
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