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Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells

BACKGROUND: Cloning vectors capable of retroviral transduction have enabled stable gene overexpression in numerous mitotic cell lines. However, the relatively small number of feasible restriction enzyme sequences in their cloning sites can hinder successful generation of overexpression constructs if...

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Detalles Bibliográficos
Autores principales:	Patel, Asmita, Muñoz, Anisleidys, Halvorsen, Katherine, Rai, Priyamvada
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298557/ https://www.ncbi.nlm.nih.gov/pubmed/22248071 http://dx.doi.org/10.1186/1472-6750-12-3

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