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Requirement of Pretone by Thromboxane A(2) for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat
Hypoxic pulmonary vasoconstriction (HPV) is physiologically important response for preventing mismatching between ventilation and perfusion in lungs. The HPV of isolated pulmonary arteries (HPV-PA) usually require a partial pretone by thromboxane agonist (U46619). Because the HPV of ventilated/perfu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Physiological Society and The Korean Society of Pharmacology
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298827/ https://www.ncbi.nlm.nih.gov/pubmed/22416221 http://dx.doi.org/10.4196/kjpp.2012.16.1.59 |
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author | Park, Su Jung Yoo, Hae Young Kim, Hye Jin Kim, Jin Kyoung Zhang, Yin-Hua Kim, Sung Joon |
author_facet | Park, Su Jung Yoo, Hae Young Kim, Hye Jin Kim, Jin Kyoung Zhang, Yin-Hua Kim, Sung Joon |
author_sort | Park, Su Jung |
collection | PubMed |
description | Hypoxic pulmonary vasoconstriction (HPV) is physiologically important response for preventing mismatching between ventilation and perfusion in lungs. The HPV of isolated pulmonary arteries (HPV-PA) usually require a partial pretone by thromboxane agonist (U46619). Because the HPV of ventilated/perfused lungs (HPV-lung) can be triggered without pretone conditioning, we suspected that a putative tissue factor might be responsible for the pretone of HPV. Here we investigated whether HPV can be also observed in precision-cut lung slices (PCLS) from rats. The HPV in PCLS also required partial contraction by U46619. In addition, K(+) channel blockers (4AP and TEA) required U46619-pretone to induce significant contraction of PA in PCLS. In contrast, the airways in PCLS showed reversible contraction in response to the K(+) channel blockers without pretone conditioning. Also, the airways showed no hypoxic constriction but a relaxation under the partial pretone by U46619. The airways in PCLS showed reliable, concentration-dependent contraction by metacholine (EC(50), ~210 nM). In summary, the HPV in PCLS is more similar to isolated PA than V/P lungs. The metacholine-induced constriction of bronchioles suggested that the PLCS might be also useful for studying airway physiology in situ. |
format | Online Article Text |
id | pubmed-3298827 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Korean Physiological Society and The Korean Society of Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-32988272012-03-13 Requirement of Pretone by Thromboxane A(2) for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat Park, Su Jung Yoo, Hae Young Kim, Hye Jin Kim, Jin Kyoung Zhang, Yin-Hua Kim, Sung Joon Korean J Physiol Pharmacol Original Article Hypoxic pulmonary vasoconstriction (HPV) is physiologically important response for preventing mismatching between ventilation and perfusion in lungs. The HPV of isolated pulmonary arteries (HPV-PA) usually require a partial pretone by thromboxane agonist (U46619). Because the HPV of ventilated/perfused lungs (HPV-lung) can be triggered without pretone conditioning, we suspected that a putative tissue factor might be responsible for the pretone of HPV. Here we investigated whether HPV can be also observed in precision-cut lung slices (PCLS) from rats. The HPV in PCLS also required partial contraction by U46619. In addition, K(+) channel blockers (4AP and TEA) required U46619-pretone to induce significant contraction of PA in PCLS. In contrast, the airways in PCLS showed reversible contraction in response to the K(+) channel blockers without pretone conditioning. Also, the airways showed no hypoxic constriction but a relaxation under the partial pretone by U46619. The airways in PCLS showed reliable, concentration-dependent contraction by metacholine (EC(50), ~210 nM). In summary, the HPV in PCLS is more similar to isolated PA than V/P lungs. The metacholine-induced constriction of bronchioles suggested that the PLCS might be also useful for studying airway physiology in situ. The Korean Physiological Society and The Korean Society of Pharmacology 2012-02 2012-02-28 /pmc/articles/PMC3298827/ /pubmed/22416221 http://dx.doi.org/10.4196/kjpp.2012.16.1.59 Text en Copyright © 2012 The Korean Physiological Society and The Korean Society of Pharmacology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Park, Su Jung Yoo, Hae Young Kim, Hye Jin Kim, Jin Kyoung Zhang, Yin-Hua Kim, Sung Joon Requirement of Pretone by Thromboxane A(2) for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat |
title | Requirement of Pretone by Thromboxane A(2) for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat |
title_full | Requirement of Pretone by Thromboxane A(2) for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat |
title_fullStr | Requirement of Pretone by Thromboxane A(2) for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat |
title_full_unstemmed | Requirement of Pretone by Thromboxane A(2) for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat |
title_short | Requirement of Pretone by Thromboxane A(2) for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat |
title_sort | requirement of pretone by thromboxane a(2) for hypoxic pulmonary vasoconstriction in precision-cut lung slices of rat |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298827/ https://www.ncbi.nlm.nih.gov/pubmed/22416221 http://dx.doi.org/10.4196/kjpp.2012.16.1.59 |
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