Cargando…
Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration
We examined the effects of bone morphogenetic protein-2 (BMP-2) on growth, differentiation, and intracellular signaling pathways of odontoblast-like cells, KN-3 cells, to clarify molecular mechanisms of odontoblast differentiation during pulp regeneration process. After treatment with BMP-2, the cel...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299296/ https://www.ncbi.nlm.nih.gov/pubmed/22505896 http://dx.doi.org/10.1155/2012/258469 |
_version_ | 1782226104070701056 |
---|---|
author | Washio, Ayako Kitamura, Chiaki Morotomi, Takahiko Terashita, Masamichi Nishihara, Tatsuji |
author_facet | Washio, Ayako Kitamura, Chiaki Morotomi, Takahiko Terashita, Masamichi Nishihara, Tatsuji |
author_sort | Washio, Ayako |
collection | PubMed |
description | We examined the effects of bone morphogenetic protein-2 (BMP-2) on growth, differentiation, and intracellular signaling pathways of odontoblast-like cells, KN-3 cells, to clarify molecular mechanisms of odontoblast differentiation during pulp regeneration process. After treatment with BMP-2, the cell morphology, growth, alkaline phosphatase (ALP) activity, and the activation and expression of BMP-induced intracellular signaling molecules, such as Smad1/5/8 and Smad6/7, as well as activities of dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP1), were examined. BMP-2 had no effects on the morphology, growth, or ALP activity of KN-3 cells, whereas it induced the phosphorylation of Smad1/5/8 and expression of Smad6/7. BMP-2 also induced the expressions of DSP and DMP-1. Our results suggest that KN-3 cells may express an odontoblastic phenotype with the addition of BMP-2 through the activation of Smad signaling pathways. |
format | Online Article Text |
id | pubmed-3299296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-32992962012-04-13 Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration Washio, Ayako Kitamura, Chiaki Morotomi, Takahiko Terashita, Masamichi Nishihara, Tatsuji Int J Dent Research Article We examined the effects of bone morphogenetic protein-2 (BMP-2) on growth, differentiation, and intracellular signaling pathways of odontoblast-like cells, KN-3 cells, to clarify molecular mechanisms of odontoblast differentiation during pulp regeneration process. After treatment with BMP-2, the cell morphology, growth, alkaline phosphatase (ALP) activity, and the activation and expression of BMP-induced intracellular signaling molecules, such as Smad1/5/8 and Smad6/7, as well as activities of dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP1), were examined. BMP-2 had no effects on the morphology, growth, or ALP activity of KN-3 cells, whereas it induced the phosphorylation of Smad1/5/8 and expression of Smad6/7. BMP-2 also induced the expressions of DSP and DMP-1. Our results suggest that KN-3 cells may express an odontoblastic phenotype with the addition of BMP-2 through the activation of Smad signaling pathways. Hindawi Publishing Corporation 2012 2012-02-28 /pmc/articles/PMC3299296/ /pubmed/22505896 http://dx.doi.org/10.1155/2012/258469 Text en Copyright © 2012 Ayako Washio et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Washio, Ayako Kitamura, Chiaki Morotomi, Takahiko Terashita, Masamichi Nishihara, Tatsuji Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
title | Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
title_full | Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
title_fullStr | Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
title_full_unstemmed | Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
title_short | Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
title_sort | possible involvement of smad signaling pathways in induction of odontoblastic properties in kn-3 cells by bone morphogenetic protein-2: a growth factor to induce dentin regeneration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299296/ https://www.ncbi.nlm.nih.gov/pubmed/22505896 http://dx.doi.org/10.1155/2012/258469 |
work_keys_str_mv | AT washioayako possibleinvolvementofsmadsignalingpathwaysininductionofodontoblasticpropertiesinkn3cellsbybonemorphogeneticprotein2agrowthfactortoinducedentinregeneration AT kitamurachiaki possibleinvolvementofsmadsignalingpathwaysininductionofodontoblasticpropertiesinkn3cellsbybonemorphogeneticprotein2agrowthfactortoinducedentinregeneration AT morotomitakahiko possibleinvolvementofsmadsignalingpathwaysininductionofodontoblasticpropertiesinkn3cellsbybonemorphogeneticprotein2agrowthfactortoinducedentinregeneration AT terashitamasamichi possibleinvolvementofsmadsignalingpathwaysininductionofodontoblasticpropertiesinkn3cellsbybonemorphogeneticprotein2agrowthfactortoinducedentinregeneration AT nishiharatatsuji possibleinvolvementofsmadsignalingpathwaysininductionofodontoblasticpropertiesinkn3cellsbybonemorphogeneticprotein2agrowthfactortoinducedentinregeneration |