Cargando…

Rapid, Specific Detection of Alphaviruses from Tissue Cultures Using a Replicon-Defective Reporter Gene Assay

We established a rapid, specific technique for detecting alphaviruses using a replicon-defective reporter gene assay derived from the Sindbis virus XJ-160. The pVaXJ expression vector containing the XJ-160 genome was engineered to form the expression vectors pVaXJ-EGFP expressing enhanced green fluo...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Jiangjiao, Zhu, Wuyang, Wang, Huanqin, Li, Jiandong, Zhang, Quanfu, He, Ying, Li, Jia, Fu, Juanjuan, Li, Dexin, Liang, Guodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299729/
https://www.ncbi.nlm.nih.gov/pubmed/22427930
http://dx.doi.org/10.1371/journal.pone.0033007