Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons

Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this...

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Autores principales: Pinto, Luísa, Mateus-Pinheiro, António, Morais, Mónica, Bessa, João Miguel, Sousa, Nuno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299750/
https://www.ncbi.nlm.nih.gov/pubmed/22427964
http://dx.doi.org/10.1371/journal.pone.0033114
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author Pinto, Luísa
Mateus-Pinheiro, António
Morais, Mónica
Bessa, João Miguel
Sousa, Nuno
author_facet Pinto, Luísa
Mateus-Pinheiro, António
Morais, Mónica
Bessa, João Miguel
Sousa, Nuno
author_sort Pinto, Luísa
collection PubMed
description Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple non-invasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders.
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spelling pubmed-32997502012-03-16 Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons Pinto, Luísa Mateus-Pinheiro, António Morais, Mónica Bessa, João Miguel Sousa, Nuno PLoS One Research Article Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple non-invasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders. Public Library of Science 2012-03-12 /pmc/articles/PMC3299750/ /pubmed/22427964 http://dx.doi.org/10.1371/journal.pone.0033114 Text en Pinto et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pinto, Luísa
Mateus-Pinheiro, António
Morais, Mónica
Bessa, João Miguel
Sousa, Nuno
Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons
title Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons
title_full Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons
title_fullStr Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons
title_full_unstemmed Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons
title_short Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons
title_sort immuno-golgi as a tool for analyzing neuronal 3d-dendritic structure in phenotypically characterized neurons
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299750/
https://www.ncbi.nlm.nih.gov/pubmed/22427964
http://dx.doi.org/10.1371/journal.pone.0033114
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