Cargando…
Evaluation of the Role of Candida albicans Agglutinin-Like Sequence (Als) Proteins in Human Oral Epithelial Cell Interactions
The fungus C. albicans uses adhesins to interact with human epithelial surfaces in the processes of colonization and pathogenesis. The C. albicans ALS (agglutinin-like sequence) gene family encodes eight large cell-surface glycoproteins (Als1-Als7 and Als9) that have adhesive function. This study ut...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299778/ https://www.ncbi.nlm.nih.gov/pubmed/22428031 http://dx.doi.org/10.1371/journal.pone.0033362 |
_version_ | 1782226170422493184 |
---|---|
author | Murciano, Celia Moyes, David L. Runglall, Manohursingh Tobouti, Priscila Islam, Ayesha Hoyer, Lois L. Naglik, Julian R. |
author_facet | Murciano, Celia Moyes, David L. Runglall, Manohursingh Tobouti, Priscila Islam, Ayesha Hoyer, Lois L. Naglik, Julian R. |
author_sort | Murciano, Celia |
collection | PubMed |
description | The fungus C. albicans uses adhesins to interact with human epithelial surfaces in the processes of colonization and pathogenesis. The C. albicans ALS (agglutinin-like sequence) gene family encodes eight large cell-surface glycoproteins (Als1-Als7 and Als9) that have adhesive function. This study utilized C. albicans Δals mutant strains to investigate the role of the Als family in oral epithelial cell adhesion and damage, cytokine induction and activation of a MAPK-based (MKP1/c-Fos) signaling pathway that discriminates between yeast and hyphae. Of the eight Δals mutants tested, only the Δals3 strain showed significant reductions in oral epithelial cell adhesion and damage, and cytokine production. High fungal:epithelial cell multiplicities of infection were able to rescue the cell damage and cytokine production phenotypes, demonstrating the importance of fungal burden in mucosal infections. Despite its adhesion, damage and cytokine induction phenotypes, the Δals3 strain induced MKP1 phosphorylation and c-Fos production to a similar extent as control cells. Our data demonstrate that Als3 is involved directly in epithelial adhesion but indirectly in cell damage and cytokine induction, and is not the factor targeted by oral epithelial cells to discriminate between the yeast and hyphal form of C. albicans. |
format | Online Article Text |
id | pubmed-3299778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32997782012-03-16 Evaluation of the Role of Candida albicans Agglutinin-Like Sequence (Als) Proteins in Human Oral Epithelial Cell Interactions Murciano, Celia Moyes, David L. Runglall, Manohursingh Tobouti, Priscila Islam, Ayesha Hoyer, Lois L. Naglik, Julian R. PLoS One Research Article The fungus C. albicans uses adhesins to interact with human epithelial surfaces in the processes of colonization and pathogenesis. The C. albicans ALS (agglutinin-like sequence) gene family encodes eight large cell-surface glycoproteins (Als1-Als7 and Als9) that have adhesive function. This study utilized C. albicans Δals mutant strains to investigate the role of the Als family in oral epithelial cell adhesion and damage, cytokine induction and activation of a MAPK-based (MKP1/c-Fos) signaling pathway that discriminates between yeast and hyphae. Of the eight Δals mutants tested, only the Δals3 strain showed significant reductions in oral epithelial cell adhesion and damage, and cytokine production. High fungal:epithelial cell multiplicities of infection were able to rescue the cell damage and cytokine production phenotypes, demonstrating the importance of fungal burden in mucosal infections. Despite its adhesion, damage and cytokine induction phenotypes, the Δals3 strain induced MKP1 phosphorylation and c-Fos production to a similar extent as control cells. Our data demonstrate that Als3 is involved directly in epithelial adhesion but indirectly in cell damage and cytokine induction, and is not the factor targeted by oral epithelial cells to discriminate between the yeast and hyphal form of C. albicans. Public Library of Science 2012-03-12 /pmc/articles/PMC3299778/ /pubmed/22428031 http://dx.doi.org/10.1371/journal.pone.0033362 Text en Murciano et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Murciano, Celia Moyes, David L. Runglall, Manohursingh Tobouti, Priscila Islam, Ayesha Hoyer, Lois L. Naglik, Julian R. Evaluation of the Role of Candida albicans Agglutinin-Like Sequence (Als) Proteins in Human Oral Epithelial Cell Interactions |
title | Evaluation of the Role of Candida albicans Agglutinin-Like Sequence (Als) Proteins in Human Oral Epithelial Cell Interactions |
title_full | Evaluation of the Role of Candida albicans Agglutinin-Like Sequence (Als) Proteins in Human Oral Epithelial Cell Interactions |
title_fullStr | Evaluation of the Role of Candida albicans Agglutinin-Like Sequence (Als) Proteins in Human Oral Epithelial Cell Interactions |
title_full_unstemmed | Evaluation of the Role of Candida albicans Agglutinin-Like Sequence (Als) Proteins in Human Oral Epithelial Cell Interactions |
title_short | Evaluation of the Role of Candida albicans Agglutinin-Like Sequence (Als) Proteins in Human Oral Epithelial Cell Interactions |
title_sort | evaluation of the role of candida albicans agglutinin-like sequence (als) proteins in human oral epithelial cell interactions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299778/ https://www.ncbi.nlm.nih.gov/pubmed/22428031 http://dx.doi.org/10.1371/journal.pone.0033362 |
work_keys_str_mv | AT murcianocelia evaluationoftheroleofcandidaalbicansagglutininlikesequencealsproteinsinhumanoralepithelialcellinteractions AT moyesdavidl evaluationoftheroleofcandidaalbicansagglutininlikesequencealsproteinsinhumanoralepithelialcellinteractions AT runglallmanohursingh evaluationoftheroleofcandidaalbicansagglutininlikesequencealsproteinsinhumanoralepithelialcellinteractions AT toboutipriscila evaluationoftheroleofcandidaalbicansagglutininlikesequencealsproteinsinhumanoralepithelialcellinteractions AT islamayesha evaluationoftheroleofcandidaalbicansagglutininlikesequencealsproteinsinhumanoralepithelialcellinteractions AT hoyerloisl evaluationoftheroleofcandidaalbicansagglutininlikesequencealsproteinsinhumanoralepithelialcellinteractions AT naglikjulianr evaluationoftheroleofcandidaalbicansagglutininlikesequencealsproteinsinhumanoralepithelialcellinteractions |