Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement

Invasion of cells from surrounding tissues is a crucial step for regeneration when using a-cellular scaffolds as a replacement of the nucleus pulposus (NP). The aim of current study was to assess whether NP and surrounding annulus fibrosus (AF) cells are capable of migrating into dense collagen scaf...

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Autores principales: Bron, Johannes L., Mulder, Harriet W., Vonk, Lucienne A., Doulabi, Bherouz Zandieh, Oudhoff, M. J., Smit, Theodoor H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299969/
https://www.ncbi.nlm.nih.gov/pubmed/22218929
http://dx.doi.org/10.1007/s10856-011-4545-7
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author Bron, Johannes L.
Mulder, Harriet W.
Vonk, Lucienne A.
Doulabi, Bherouz Zandieh
Oudhoff, M. J.
Smit, Theodoor H.
author_facet Bron, Johannes L.
Mulder, Harriet W.
Vonk, Lucienne A.
Doulabi, Bherouz Zandieh
Oudhoff, M. J.
Smit, Theodoor H.
author_sort Bron, Johannes L.
collection PubMed
description Invasion of cells from surrounding tissues is a crucial step for regeneration when using a-cellular scaffolds as a replacement of the nucleus pulposus (NP). The aim of current study was to assess whether NP and surrounding annulus fibrosus (AF) cells are capable of migrating into dense collagen scaffolds. We seeded freshly harvested caprine NP and AF cells onto scaffolds consisting of 1.5 and 3.0% type I collagen matrices, prepared by plastic compression, to assess cell invasion. The migration distance appeared both time and density dependent and was higher for NP (25%) compared to AF (10%) cells after 4 weeks. Migration distance was not enhanced by Hst-2, a peptide derived from saliva known to enhance fibroblast migration, and this was confirmed in a scratch assay. In conclusion, we revealed invasion of cells into dense collagen scaffolds and therewith encouraging first steps towards the use of a-cellular scaffolds for NP replacement.
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spelling pubmed-32999692012-03-22 Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement Bron, Johannes L. Mulder, Harriet W. Vonk, Lucienne A. Doulabi, Bherouz Zandieh Oudhoff, M. J. Smit, Theodoor H. J Mater Sci Mater Med Article Invasion of cells from surrounding tissues is a crucial step for regeneration when using a-cellular scaffolds as a replacement of the nucleus pulposus (NP). The aim of current study was to assess whether NP and surrounding annulus fibrosus (AF) cells are capable of migrating into dense collagen scaffolds. We seeded freshly harvested caprine NP and AF cells onto scaffolds consisting of 1.5 and 3.0% type I collagen matrices, prepared by plastic compression, to assess cell invasion. The migration distance appeared both time and density dependent and was higher for NP (25%) compared to AF (10%) cells after 4 weeks. Migration distance was not enhanced by Hst-2, a peptide derived from saliva known to enhance fibroblast migration, and this was confirmed in a scratch assay. In conclusion, we revealed invasion of cells into dense collagen scaffolds and therewith encouraging first steps towards the use of a-cellular scaffolds for NP replacement. Springer US 2012-01-05 2012 /pmc/articles/PMC3299969/ /pubmed/22218929 http://dx.doi.org/10.1007/s10856-011-4545-7 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Bron, Johannes L.
Mulder, Harriet W.
Vonk, Lucienne A.
Doulabi, Bherouz Zandieh
Oudhoff, M. J.
Smit, Theodoor H.
Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement
title Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement
title_full Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement
title_fullStr Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement
title_full_unstemmed Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement
title_short Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement
title_sort migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299969/
https://www.ncbi.nlm.nih.gov/pubmed/22218929
http://dx.doi.org/10.1007/s10856-011-4545-7
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